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首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >Isolation of polysaccharides from Malva aegyptiaca and evaluation of their antioxidant and antibacterial properties
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Isolation of polysaccharides from Malva aegyptiaca and evaluation of their antioxidant and antibacterial properties

机译:来自马尔尔夫艾·埃比亚的多糖分离,评价其抗氧化剂和抗菌性能

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This study investigated the antioxidant and antibacterial properties of crude water-soluble polysaccharides extracted from the mallow (Malva aegyptiaca) by precipitation with cetylpyridinium chloride (P1) or ethanol (P2). The Polysaccharides fractions were characterized by FT1R spectroscopy, a monosaccharide composition, and antioxidant and antibacterial activities. P1 showed the highest total sugars (81.2%) and sulfated groups (2.9%) contents but with the lowest total proteins content (8.7%). The infrared spectroscopic spectra exhibited the typical bands and peak characteristic of polysaccharides. Monosaccharides analysis revealed the dominance of galactose (40.3-43.7%) and glucuronic acid (25.9-30.9%). Interestingly, P1 displayed an important antioxidant activity as evaluated by the (Fe2+) chelating activity (IC50 = 1.15 mg/ml), (Fe3+) reducing power (EC50 = 1.22 mg/ml), beta-carotene bleaching inhibition capacity (IC50 = 1.56 mg/ml) and DPPH center dot-radical scavenging activity (IC50 = 1.94 mg/ml). Furthermore, P1 at 10 mg/ml was highly active than P2 against several bacterial strains and especially the Gram-positive bacteria, where the inhibition rate ranged between 84.2 and 90.3%. Obtained results suggest that M. aegyptiaca polysaccharides precipitated by cetylpyridinium chloride could be used as a naural antioxidant and antibacterial agent. (C) 2017 Elsevier B.V. All rights reserved.
机译:本研究研究了通过沉淀与氯化甲吡啶(P1)或乙醇(P2)沉淀从冬葵(MALVA AEGYPIACA)中提取的粗水溶性多糖的抗氧化剂和抗菌性质。通过FT1R光谱,单糖组合物和抗氧化剂和抗菌活性表征多糖级分。 P1显示最高糖(81.2%)和硫酸盐基团(2.9%)含量,但总蛋白质含量最低(8.7%)。红外光谱光谱表现出多糖的典型带和峰特征。单糖分析显示半乳糖(40.3-43.7%)和葡糖醛酸(25.9-30.9%)的优势。有趣的是,P1显示了由(Fe2 +)螯合活性(IC50 = 1.15mg / ml)评估的重要抗氧化活性,(Fe3 +)还原功率(EC50 = 1.22mg / ml),β-胡萝卜素漂白抑制容量(IC50 = 1.56 Mg / ml)和DPPH中心点 - 自由基清除活性(IC50 = 1.94mg / ml)。此外,10mg / ml的P1比对于几种细菌菌株,尤其是革兰氏阳性细菌的P1高,抑制率在84.2至90.3%之间。得到的结果表明,由十六烷基吡啶氯化吡啶沉淀的M.Agypiaca多糖可用作Naural抗氧化剂和抗菌剂。 (c)2017年Elsevier B.V.保留所有权利。

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