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Recovery and utilization of collagen protein powder extracted from chromium leather scrap waste

机译：从铬皮革废料中提取的胶原蛋白粉末的恢复和利用

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摘要

In this work, we investigate collagen protein powder (CPP) extracted from chromium leather scrap waste (CLSW). The composition and molecular weight distribution of CPP were determined by elemental analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), respectively. The microstructure and size distribution of CPP were then characterized by scanning electron microscopy (SEM) and nanometer analyzer instrument. Finally, CPP was treated with corn starch (CS), and the swelling behavior of the resulting CPP-CS blend was investigated in order to determine its range of applications. The experimental data showed that CPP contains 13 different amino-acids. CPP also displayed low mineral salt levels and a nitrogen content of 43.84%, indicating its potential use as an organic fertilizer. The molecular weight range of CPP is 6.5 to similar to 26.6 kDa. After the obtained CPP was blended with CS, the CPP-CS blend is endowed with optimal swelling properties and is able to overcome the solubility drawbacks of CPP alone. In addition, when the CPP was used as a natural fertilizer, the germination rate and height of kidney beans obviously increased.
机译：在这项工作中，我们研究了从铬皮革废料（CLSW）中提取的胶原蛋白粉末（CPP）。 CPP的组成和分子量分布通过元素分析和十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（SDS-PAGE）测定。然后通过扫描电子显微镜（SEM）和纳米分析仪来表征CPP的微观结构和尺寸分布。最后，CPP用玉米淀粉（CS）处理，并研究了所得CPP-CS混合物的溶胀行为，以确定其应用范围。实验数据显示CPP含有13种不同的氨基酸。 CPP还显示出低的矿物盐水平和氮含量为43.84％，表明其潜在用作有机肥料。 CPP的分子量范围为6.5至类似于26.6kDa。在将获得的CPP与CS混合后，CPP-CS混合物具有最佳的溶胀性能，并且能够克服单独的CPP的溶解性缺点。此外，当CPP用作天然肥料时，芸豆的发芽率和高度明显增加。

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