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首页> 外文期刊>Environmental Science and Pollution Research >Laboratory bioassays on the response of honey bee (Apis mellifera L.) glutathione S-transferase and acetylcholinesterase to the oral exposure to copper, cadmium, and lead
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Laboratory bioassays on the response of honey bee (Apis mellifera L.) glutathione S-transferase and acetylcholinesterase to the oral exposure to copper, cadmium, and lead

机译：蜜蜂（Apis Mellifera L.）谷胱甘肽S-转移酶和乙酰胆碱酯酶对铜，镉和铅的响应的实验室生物测量

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In the present study, the influence of cadmium, copper, and lead on two enzymes often used as biomarkers in toxicological analysis was investigated. Bees were fed with 1M sucrose solution containing 10-fold serial dilutions of CuCl2 (1000mgL(-1), 100mgL(-1), and 10mgL(-1)), CdCl2 (0.1mgL(-1), 0.01mgL(-1), and 0.001mgL(-1)), or PbCl2 (10mgL(-1), 1mgL(-1), and 0.1mgL(-1)) during 48h. Our results showed that the total glutathione S-transferase activity was not changed under the influence of cadmium and lead, and it was decreased with the highest concentration of copper. The level of gene expression of the three analyzed classes of glutathione S-transferase was significantly increased with increasing concentrations of copper and cadmium. Lead did not cause significant changes in glutathione S-transferase activity and gene expression, while it showed biphasic effect on acetylcholinesterase activity: lower concentration of lead, 0.1mgL(-1) inhibited and higher dose, 10mgL(-1) induced acetylcholinesterase activity in honey bees. Furthermore, our results showed a significant decrease of the acetylcholinesterase activity in honey bees treated with 0.001 and 0.01mgL(-1) CdCl2. Our results indicate the influence of cadmium, copper, and lead on GST and AChE in the honey bees. These results form the basis for future research on the impact of metallic trace element pollution on honey bees.
机译：在本研究中，研究了镉，铜和铅对毒理学分析中的两种酶的影响。用1M蔗糖溶液喂食含有10倍的CuCl 2（1000mgL（-1），100mgL（-1）和10mgL（-1）），CdCl 2（0.1mg1），0.01mg1（-1在48h期间，0.001mg1（-1）），或0.001mg1（-1））或PbCl 2（10mgl（-1），1mg1（-1）和0.1mg1（-1））。我们的研究结果表明，在镉和铅的影响下，谷胱甘肽S转移酶活性不会改变，并且随着铜的最高浓度降低。随着铜和镉的浓度，三种分析的谷胱甘肽S-转移酶的基因表达水平显着增加。铅没有造成显着变化的谷胱甘肽S-转移酶活性和基因表达，同时它表现出对乙酰胆碱酯酶活性的双色作用：较低浓度的铅，0.1mg1（-1）抑制且较高剂量，10mgL（-1）诱导乙酰胆碱酯酶活性蜜蜂。此外，我们的结果表明，用0.001和0.01mg1（-1）CDCl2处理的蜂蜜蜜蜂中乙酰胆碱酯酶活性显着降低。我们的结果表明镉，铜，铅对蜂蜜蜜蜂的GST和疼痛的影响。这些结果构成了未来研究金属微量元素污染对蜂蜜蜜蜂的影响的研究的基础。

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