...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Environmental Science and Pollution Research >Chromobacterium violaceum UTM5 against Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA)]]>
【24h】

Chromobacterium violaceum UTM5 against Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA)]]>

机译：<！[cdata [virtapation的抗菌作用模式来自<重点型=“斜体”>富集型紫杉杆菌 UTM5，反对<重点型=“斜体”>金属葡萄球菌和甲氧西林抗性<重点类型= “斜体”>金黄色葡萄球菌（MRSA）]]>

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

AbstractViolacein, violet pigment produced byChromobacterium violaceum, has attracted much attention recently due to its pharmacological properties including antibacterial activity. The present study investigated possible antibacterial mode of action of violacein fromC. violaceumUTM5 againstStaphylococcus aureusand methicillin-resistantS. aureus(MRSA) strains. Violet fraction was obtained by cultivatingC. violaceumUTM5 in liquid pineapple waste medium, extracted, and fractionated using ethyl acetate and vacuum liquid chromatography technique. Violacein was quantified as major compound in violet fraction using HPLC analysis. Violet fraction displayed bacteriostatic activity againstS. aureusATCC 29213 and methicillin-resistantS. aureusATCC 43300 with minimum inhibitory concentration (MIC) of 3.9?μg/mL. Fluorescence dyes for membrane damage and scanning electron microscopic analysis confirmed the inhibitory effect by disruption on membrane integrity, morphological alternations, and rupture of the cell membranes of both strains. Transmission electron microscopic analysis showed membrane damage, mesosome formation, and leakage of intracellular constituents of both bacterial strains. Mode of action of violet fraction on the cell membrane integrity of both strains was shown by release of protein, K+, and extracellular adenosine 5′-triphosphate (ATP) with 110.5?μg/mL, 2.34?μg/mL, and 87.24?ng/μL, respectively, at 48?h of incubation. Violet fraction was toxic to human embryonic kidney (HEK293) and human fetal lung fibroblast (IMR90) cell lines with LC50value of 0.998?±?0.058 and 0.387?±?0.002?μg/mL, respectively. Thus, violet fraction showed a strong antibacterial property by disrupting the membrane integrity ofS. aureusand MRSA strains. This is the first report on the possible mode of antibacterial action of violet fraction fromC. violaceumUTM5 onS. aureusand MRSA strains.]]>
机译：<！[CDATA [<摘要ID =“abs1”语言=“en”outputmedium =“全部”> <标题>抽象 ara id =“par1”> violetein，由<重点类型=“ Italic“> Chofobacterium ，由于其药理学性质，并且在包括抗菌活性的药理学性质引起了很多关注。本研究调查了来自<重点型=“斜体”> C的紫杉醇的可能抗菌作用模式。 violaceum UTM5对<重点类型=“斜体”>金黄色葡萄球菌和Methicililin抗性<重点类型=“斜体”> s。金黄色葡萄球菌（MRSA）菌株。通过培养<强调型=“斜体”> C获得紫级分。 violaceum UTM5在液体菠萝废料介质中，用乙酸乙酯和真空液相色谱技术提取和分级。使用HPLC分析量为紫色级分中的主要化合物量化。紫罗兰分数展示了对<重点类型=“斜体”> s的抑菌活动。 AUREUS ATCC 29213和甲氧西林抗性<重点型=“斜体”> s。 AUREUS ATCC 43300，最小抑制浓度（MIC）为3.9≤μg/ mL。用于膜损伤和扫描电子显微镜分析的荧光染料证实了通过对两种菌株的细胞膜破裂的破坏来证实了抑制作用。透射电子显微镜分析显示出膜损伤，细胞体形成和细菌菌株细胞内成分的泄漏。通过释放蛋白质，k <上标> + 和细胞外腺苷5'-三磷酸（ATP），显示两种菌株对两种菌株的细胞膜完整性的作用方式。（ATP），110.5μg/ ml，2.34？ μg/ ml，和87.24·ng /μl，在孵育48Ωh时。紫级分毒性对人胚胎肾（HEK293）和人胎肺成纤维细胞（IMR90）细胞系，LC <下标> 50 分别为0.998≤0.058和0.387？±0.002ΩΩ/ ml 。因此，通过破坏<重点型=“斜体”> s的膜完整性，紫级分数显示出强烈的抗菌性能。金黄色葡萄球菌和MRSA菌株。这是关于紫杉型=“斜体”> c紫罗兰分数的可能模式的第一个报告。 violaceum UTM5 on s。金黄色葡萄球菌和MRSA菌株。 ]]>

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号