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Proteomic analysis of continuous 900-MHz radiofrequency electromagnetic field exposure in testicular tissue: a rat model of human cell phone exposure

机译:睾丸组织连续900-MHz射频电磁场暴露的蛋白质组学分析:人体手机暴露的大鼠模型

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摘要

Although cell phones have been used worldwide, some adverse and toxic effects were reported for this communication technology apparatus. To analyze in vivo effects of exposure to radiofrequency-electromagnetic field (RF-EMF) on protein expression in rat testicular proteome, 20 Sprague-Dawley rats were exposed to 900 MHz RF-EMF for 0, 1, 2, or 4 h/day for 30 consecutive days. Protein content of rat testes was separated by high-resolution two-dimensional electrophoresis using immobilized pH gradient (pI 4-7, 7 cm) and 12% acrylamide and identified by MALDI-TOF/TOF-MS. Two protein spots were found differentially overexpressed (P < 0.05) in intensity and volume with induction factors 1.7 times greater after RF-EMF exposure. After 4 h of daily exposure for 30 consecutive days, ATP synthase beta subunit (ASBS) and hypoxia up-regulated protein 1 precursor (HYOU1) were found to be significantly up-regulated. These proteins affect signaling pathways in rat testes and spermatogenesis and play a critical role in protein folding and secretion in the endoplasmic reticulum. Our results indicate that exposure to RF-EMF produces increases in testicular proteins in adults that are related to carcinogenic risk and reproductive damage. In light of the widespread practice of men carrying phones in their pockets near their gonads, where exposures can exceed as-tested guidelines, further study of these effects should be a high priority.
机译:尽管在全球范围内使用了手机,但据报道了这种通信技术设备的一些不利和有毒的效果。为了分析暴露于射频 - 电磁场(RF-EMF)对大鼠睾丸蛋白质组的蛋白质表达的体内影响,将20只Sprague-Dawley大鼠暴露于900MHz的RF-EMF,0,1,2或4 H /天连续30天。通过使用固定化的pH梯度(PI 4-7,7cm)和12%丙烯酰胺通过高分辨率二维电泳分离大鼠睾丸蛋白质含量,并由MALDI-TOF / TOF-MS鉴定。在RF-EMF暴露后,在强度和体积中发现两种蛋白质斑点差异和体积差异,具有诱导因子1.7倍。在连续30天进行每日暴露4小时后,发现ATP合酶β亚基(ASB)和缺氧上调蛋白1前体(HYOU1)明显上调。这些蛋白质影响大鼠测试和精子发生中的信号通路,并在内质网的蛋白质折叠和分泌中发挥关键作用。我们的结果表明,接触RF-EMF会产生与致癌风险和生殖损伤有关的成年人睾丸蛋白质的增加。鉴于男子携带手机的广泛实践,在他们的GONAD附近的口袋里,曝光可能超过经过测试的准则,进一步研究这些效果应该是一个高优先级。

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