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首页> 外文期刊>Molecular medicine reports >Icariin promotes stable chondrogenic differentiation of bone marrow mesenchymal stem cells in self-assembling peptide nanofiber hydrogel scaffolds
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Icariin promotes stable chondrogenic differentiation of bone marrow mesenchymal stem cells in self-assembling peptide nanofiber hydrogel scaffolds

机译：icariin在自组装肽纳米纤维水凝胶支架中促进骨髓间充质干细胞的稳定软骨分化

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摘要

Icariin, a traditional Chinese medicine, has previously been demonstrated to promote chondrogenesis of bone marrow mesenchymal stem cells (BMSCs) in traditional 2D cell culture. The present study investigated whether icariin has the potential to promote stable chondrogenic differentiation of BMSCs without hypertrophy in a 3D microenvironment. BMSCs were cultivated in a self-assembling peptide nanofiber hydrogel scaffold in chondrogenic medium for 3 weeks. Icariin was added to the medium throughout the culture period at concentrations of 1x10(-6) M. Chondrogenic differentiation markers, including collagen II and SRY-type high mobility group box 9 (SOX9) were detected by immunofluorescence, reverse transcription-quantitative polymerase chain reaction and toluidine blue staining. Hypertrophic differentiation was further assessed by detecting collagen X and collagen I gene expression levels and alkaline phosphatase activity. The results demonstrated that icariin significantly enhanced cartilage extracellular matrix synthesis and gene expression levels of collagen II and SOX9, and additionally promoted more chondrocyte-like rounded morphology in BMSCs. Furthermore, chondrogenic medium led to hypertrophic differentiation via upregulation of collagen X and collagen I gene expression levels and alkaline phosphatase activity, which was not potentiated by icariin. In conclusion, these results suggested that icariin treatment may promote chondrogenic differentiation of BMSCs, and inhibit the side effect of growth factor activity, thus preventing further hypertrophic differentiation. Therefore, icariin may be a potential compound for cartilage tissue engineering.
机译：中医伊加里宁先前已经证明，在传统的2D细胞培养中促进骨髓间充质干细胞（BMSC）的软骨菌。本研究调查了icariin是否有可能在3D微环境中促进BMSCs的稳定软弱化学分化。在软骨培养基中在软组装肽纳米纤维水凝胶支架中培养BMSC 3周。通过免疫荧光，逆转录定量聚合酶链检测胰蛋白浓度为1×10（-6）M.浓度为1×10（-6）米的培养周期内的培养基中加入培养基中，包括胶原II和Sry型高迁移率组箱9（SOX9）反应和甲苯胺蓝染色。通过检测胶原X和胶原I基因表达水平和碱性磷酸酶活性，进一步评估肥厚分化。结果表明，伊加里宁显着增强了软骨细胞外基质合成和基因表达水平的胶原II和SOX9，并且另外促进了BMSCs中的更多软骨细胞样圆形形态。此外，软骨培养基通过胰蛋白酶未加强的胶原X和胶原蛋白I基因表达水平和碱性磷酸酶活性而导致肥厚分化。总之，这些结果表明，伊加里宁治疗可以促进BMSCs的软骨性分化，并抑制生长因子活性的副作用，从而防止进一步的肥厚分化。因此，伊加里宁可以是软骨组织工程的潜在化合物。

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