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首页> 外文期刊>Molecular medicine reports >Role of ataxia-telangiectasia mutated in hydrogen peroxide preconditioning against oxidative stress in Neuro-2a cells
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Role of ataxia-telangiectasia mutated in hydrogen peroxide preconditioning against oxidative stress in Neuro-2a cells

机译：非亚氧化物在神经2A细胞中氧化胁迫下过氧化氢致氧化的作用

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摘要

Ischemic preconditioning is an endogenous protective mechanism that may be triggered by exposure to hydrogen peroxide (H2O2). However, the exact mechanisms underlying preconditioning remain to be fully understood. Ataxia-telangiectasia mutated (ATM) is regarded as an essential endogenous protective protein against stress. The aim of the present study was therefore to investigate whether ATM mediates H2O2 preconditioning. Preconditioning of Neuro-2a (N2a) cells with 100 mu M H2O2 for 90 min resulted in protection from injury induced by a long period of exposure to 600 mu M H2O2. In addition, preconditioning with 100 mu M H2O2 activated ATM and increased ATM mRNA and protein expression levels in N2a cells. Furthermore, the protective effects induced by H2O2 preconditioning were attenuated by pretreatment with the ATM inhibitor, Ku55933, or ATM small interfering RNA. In conclusion, these findings suggested that ATM is involved in H2O2 preconditioning-mediated protection against oxidative stress-induced injury in N2a cells. To the best of our knowledge, the present study demonstrated, for the first time, that the ATM protein is a key mediator of H2O2 preconditioning.
机译：缺血预处理是内源性保护机制，其可以通过暴露于过氧化氢（H2O2）而引发。然而，依据预处理的确切机制仍然被完全理解。 Ataxia-Telanciectasia突变（ATM）被认为是反对应激的基本内源性保护蛋白。因此，本研究的目的是调查ATM是否介导H2O2预处理。 Neuro-2a（N2A）细胞的预处理为100μmH2O2，得到90分钟，导致通过长时间暴露于600μmH2O2的损伤保护。此外，预处理用100μmH2O2活化的ATM和N 2 C细胞中增加的ATM mRNA和蛋白质表达水平。此外，通过用ATM抑制剂，Ku55933或ATM小干扰RNA预处理，通过预处理诱导H 2 O 2预处理的保护作用。总之，这些研究结果表明，ATM参与了H2O2预处理介导的保护免受N2A细胞中氧化应激诱导的损伤的保护。据我们所知，本研究首次证明了ATM蛋白是H2O2预处理的关键介质。

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  • 来源
    《Molecular medicine reports》 |2017年第2期|共6页
  • 作者

    Wu Jianhua; Wang Fang; Su Zhiqiang; Liu Jue; Hu Sang; Li Hao; Hu Pei; Wu Dongfang;

  • 作者单位

    Wuhan Univ Zhongnan Hosp Dept Pharm 169 Dong Hu Rd Wuhan 430071 Hubei Peoples R China;

    Wuhan Univ Zhongnan Hosp Dept Pharm 169 Dong Hu Rd Wuhan 430071 Hubei Peoples R China;

    Guangzhou Univ Tradit Chinese Med Affiliated Hosp 1 Dept Pharm Guangzhou 510405 Guangdong;

    Huazhong Univ Sci &

    Technol Tongji Med Coll Cent Hosp Wuhan Dept Pharm Wuhan 430014 Hubei;

    Wuhan Univ Zhongnan Hosp Dept Pharm 169 Dong Hu Rd Wuhan 430071 Hubei Peoples R China;

    Wuhan Univ Zhongnan Hosp Dept Pharm 169 Dong Hu Rd Wuhan 430071 Hubei Peoples R China;

    Wuhan Univ Zhongnan Hosp Dept Pharm 169 Dong Hu Rd Wuhan 430071 Hubei Peoples R China;

    Wuhan Univ Zhongnan Hosp Dept Pharm 169 Dong Hu Rd Wuhan 430071 Hubei Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 基础医学;
  • 关键词

    ataxia-telangiectasia mutated; preconditioning; hydrogen peroxide;

    机译：Ataxia-Telanciectasia突变;预处理;过氧化氢;

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