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首页> 外文期刊>Molecular medicine reports >Fluid shear stress induces osteoblast differentiation and arrests the cell cycle at the G0 phase via the ERK1/2 pathway
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Fluid shear stress induces osteoblast differentiation and arrests the cell cycle at the G0 phase via the ERK1/2 pathway

机译：流体剪切应力诱导成骨细胞分化并通过ERK1 / 2途径在G0相处捕集细胞周期

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摘要

Numerous studies have demonstrated that fluid shear stress (FSS) may promote the proliferation and differentiation of osteoblast cells. However, proliferation and differentiation are mutually exclusive processes and are unlikely to be promoted by FSS simultaneously. Cell proliferation and differentiation induced by FSS has rarely been reported. In order to provide an insight into this process, the present study investigated the effects of FSS on osteoblast-like MC3T3 cells in the G0/G1 phase, the period during which the fate of a cell is determined. The results of the present study demonstrated that FSS promoted alkaline phosphatase (ALP) activity, and the mRNA expression and protein expression of osteocalcin, collagen type I and runt-related transcription factor 2 (Runx2), while inhibiting DNA synthesis and arresting the cell cycle at the G0/G1 phase. The increase in Runx2 and ALP activity was accompanied by the activation of calcium/calmodulin-dependent protein kinase type II (CaMK II) and extracellular signal-regulated kinases 1/2 (ERK1/2), which was completely abolished by treatment with KN93 and U0126, respectively. In addition, the inhibition of ERK1/2, although not CaMK II, decreased p21(Cip/Kip) activity, resulting in an increase in cell number and S phase re-entry. The results of the present study indicated that in the G0/G1 phase, FSS promoted osteoblast differentiation via the CaMK II and ERK1/2 signaling pathways, and blocked the cell cycle at the G0/G1 phase via the ERK1/2 pathway only. The present findings provided an increased understanding of osteoblastic mechanobiology.
机译：许多研究表明，流体剪切应力（FSS）可以促进成骨细胞的增殖和分化。然而，增殖和分化是相互排斥的过程，并且不太可能同时由FSS促进。据报道，FSS诱导的细胞增殖和分化很少。为了提供对该过程的洞察力，本研究研究了FSS对G0 / G1相中的骨细胞样MC3T3细胞的影响，确定了细胞的命运的时间。本研究的结果证明，FSS促进了碱性磷酸酶（ALP）活性，以及​​骨钙素，胶原型I和RUNT相关转录因子2（RUNX2）的mRNA表达和蛋白表达，同时抑制DNA合成并捕获细胞周期在G0 / G1相位。 Runx2和AlP活性的增加伴随着钙/钙调蛋白依赖性蛋白激酶II型（CAMK II）和细胞外信号调节激酶1/2（ERK1 / 2）的激活，其通过KN93和HARE处理完全废除U0126分别。此外，抑制ERK1 / 2，尽管不是CAMK II，但P21（CIP / KIP）活性降低，导致细胞数和S相重新入口增加。本研究的结果表明，在G0 / G1相中，FSS通过CAMK II和ERK1 / 2信号传导途径促进了成骨细胞分化，并仅通过ERK1 / 2通路阻断了G0 / G1相处的细胞循环。本研究结果提供了对骨细胞机制的了解增加。

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  • 来源
    《Molecular medicine reports》 |2017年第2期|共10页
  • 作者

    Yu Liyin; Ma Xingfeng; Sun Junqin; Tong Jie; Shi Liang; Sun Lijun; Zhang Jianbao;

  • 作者单位

    Xi An Jiao Tong Univ Sch Life Sci &

    Technol Minist Educ Key Lab Biomed Informat Engn 541 Xian;

    Xi An Jiao Tong Univ Sch Life Sci &

    Technol Minist Educ Key Lab Biomed Informat Engn 541 Xian;

    Xi An Jiao Tong Univ Sch Life Sci &

    Technol Minist Educ Key Lab Biomed Informat Engn 541 Xian;

    Xi An Jiao Tong Univ Sch Life Sci &

    Technol Minist Educ Key Lab Biomed Informat Engn 541 Xian;

    Xi An Jiao Tong Univ Sch Life Sci &

    Technol Minist Educ Key Lab Biomed Informat Engn 541 Xian;

    Shaanxi Normal Univ Inst Sports Biol Xian 710119 Shaanxi Peoples R China;

    Xi An Jiao Tong Univ Sch Life Sci &

    Technol Minist Educ Key Lab Biomed Informat Engn 541 Xian;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 基础医学;
  • 关键词

    fluid shear stress; osteoblast; cell differentiation; cell cycle;

    机译：流体剪切应力;成骨细胞;细胞分化;细胞周期;

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