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首页> 外文期刊>Nanotechnology >Protein corona formation and its constitutional changes on magnetic nanoparticles in serum featuring a polydehydroalanine coating: effects of charge and incubation conditions
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Protein corona formation and its constitutional changes on magnetic nanoparticles in serum featuring a polydehydroalanine coating: effects of charge and incubation conditions

机译：蛋白质电晕形成及其对血清磁性纳米粒子的构成变化，具有多醛铝涂层：电荷和孵育条件的影响

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摘要

The inevitable formation of a protein corona upon contact of nanoparticles with different biological fluids is of great interest in the context of biomedical applications. It is well established that the surface chemistry of the respective nanomaterial has tremendous impact on protein adsorption, both in terms of the actual amount as well as the type of proteins adsorbed. In that regard, especially polyzwitterions are discussed as coating materials as they are known to partially inhibit protein adsorption. We herein present comparative incubation studies on iron oxide nanoparticles (either single core (SPION) or multicore nanoparticles (MCNP)) after coating with either polyanionic or polyzwitterionic polymeric shells based on polydehydroalanine (PDha). Apart from varying surface charge and chemistry, also the influence of incubation time and temperature on the formation and composition of a protein corona upon exposure to fetal calf serum was investigated. The amounts of adsorbed biomolecules were determined using thermogravimetric analysis. SDS-PAGE experiments revealed information on protein composition as major components of the biomolecule corona. Our results show that distinctly lower amounts of proteins are adsorbed onto polyzwitterionic hybrid nanoparticles in general, but also the corona composition varies as indicated by elevated relative ratios of medium molecular weight proteins (i.e. proteins 25-100 kDa) estimated by non-specific silver protein staining. In addition, increasing relative amounts of albumin (67 kDa) via specific Western blot assays on PDha-coated MCNP are detected.
机译：在具有不同生物流体的纳米颗粒接触时，蛋白质电晕的不可避免的形成对生物医学应用的背景有很大兴趣。很好地确定，各自纳米材料的表面化学对蛋白质吸附产生巨大影响，两者都在实际量以及吸附的蛋白质类型。在这方面，特别是聚撇管被讨论为涂料，因为已知部分地抑制蛋白质吸附。在本文中，在涂覆氧化铁纳米粒子（单芯（SpiON）或多核纳米颗粒（MCNP））的比较孵育研究，其基于多醛胺（PDHA）涂覆涂覆后涂覆涂覆后的多烷基或多种离子聚合物壳。除了不同的表面电荷和化学外，还研究了在暴露于胎牛血清时孵育时间和温度对蛋白质电晕的形成和组成的影响。使用热重分析测定吸附的生物分子的量。 SDS-PAGE实验揭示了蛋白质组合物作为生物分子电晕的主要成分的信息。我们的结果表明，较低量的蛋白质通常被吸收到聚乙酸杂交纳米颗粒上，但是电晕组合物也随由非特异性银蛋白估计的培养基分子量蛋白（即蛋白质25-100kDa）的相对比例而变化染色。另外，检测通过在PDHA涂覆的MCNP上通过特异性蛋白质印迹测定增加白蛋白（67kDa）的相对量。

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  • 来源
    《Nanotechnology》 |2019年第26期|共15页
  • 作者

    Graefe Christine; von der Luehe Moritz; Weidner Andreas; Globig Philipp; Clement Joachim H.; Dutz Silvio; Schacher Felix H.;

  • 作者单位

    Univ Klinikum Jena Klin Innere Med 2 Abt Hamatol &

    Internist Onkol Klinikum 1 D-07747 Jena Germany;

    Friedrich Schiller Univ Jena Inst Organ Chem &

    Macromol Chem IOMC Humboldtstr 10 D-07743 Jena Germany;

    Tech Univ Ilmenau Inst Biomed Engn &

    Informat Gustav Kirchhoff Str 2 D-98693 Ilmenau Germany;

    Univ Klinikum Jena Klin Innere Med 2 Abt Hamatol &

    Internist Onkol Klinikum 1 D-07747 Jena Germany;

    Univ Klinikum Jena Klin Innere Med 2 Abt Hamatol &

    Internist Onkol Klinikum 1 D-07747 Jena Germany;

    Tech Univ Ilmenau Inst Biomed Engn &

    Informat Gustav Kirchhoff Str 2 D-98693 Ilmenau Germany;

    Friedrich Schiller Univ Jena Inst Organ Chem &

    Macromol Chem IOMC Humboldtstr 10 D-07743 Jena Germany;

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  • 正文语种 eng
  • 中图分类 特种结构材料;
  • 关键词

    magnetic nanoparticles; protein corona; biomolecule corona;

    机译：磁性纳米粒子;蛋白质电晕;生物分子电晕;

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