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首页> 外文期刊>Nanotechnology >Anti-G250 nanobody-functionalized nanobubbles targeting renal cell carcinoma cells for ultrasound molecular imaging
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Anti-G250 nanobody-functionalized nanobubbles targeting renal cell carcinoma cells for ultrasound molecular imaging

机译：抗G250纳米型官能化纳米泡靶向肾细胞癌细胞，用于超声分子成像

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摘要

Traditional imaging examinations have difficulty in identifying benign and malignant changes in renal masses. This difficulty may be solved by ultrasound molecular imaging based on targeted nanobubbles, which could specifically enhance the ultrasound imaging of renal cell carcinomas (RCC) so as to discriminate benign and malignant renal masses. In this study, we aimed to prepare anti-G250 nanobody-functionalized targeted nanobubbles (anti-G250 NTNs) by coupling anti-G250 nanobodies to lipid nanobubbles and to verify their target specificity and binding ability to RCC cells that express G250 antigen and their capacity to enhance ultrasound imaging of RCC xenografts. Anti-G250 nanobodies were coupled to the lipid nanobubbles using the biotin-streptavidin bridge method. The average particle diameter of the prepared anti-G250 NTNs was 446 nm. Immunofluorescence confirmed that anti-G250 nanobodies were uniformly distributed on the surfaces of nanobubbles. In vitro experiments showed that the anti-G250 NTNs specifically bound to G250-positive 786-O cells and HeLa cells with affinities of 88.13% +/- 4.37% and 71.8% +/- 5.7%, respectively, and that they did not bind to G250-negative ACHN cells. The anti-G250 NTNs could significantly enhance the ultrasound imaging of xenograft tumors arising from 786-O cells and HeLa cells compared with blank nanobubbles, while the enhancement was not significant for xenograft tumors arising from ACHN cells. Immunofluorescence of tumor tissue slices confirmed that the anti-G250 NTNs could enter the tissue space through tumor blood vessels and bind to tumor cells specifically. In conclusion, anti-G250 nanobody-functionalized targeted nanobubbles could specifically bind to G250-positive RCC cells and enhance the ultrasound imaging of G250-positive RCC xenografts. This study has high-potential clinical application value for the diagnosis and differential diagnosis of renal tumors.
机译：传统的成像考试难以识别肾群众的良性和恶性变化。这种难题可以通过基于靶向纳米泡的超声分子成像来解决，其可以特别提高肾细胞癌（RCC）的超声成像，以区分良性和恶性肾肿块。在这项研究中，我们旨在通过将抗G250纳米胚层与脂质纳米脱发偶联并验证表达G250抗原及其能力的RCC细胞的靶特异性和结合能力来制备抗G250纳米级官能化靶纳米泡（抗G250NTN）。增强RCC异种移植物的超声成像。使用生物素 - 链霉抗生物素蛋白桥法偶联抗G250纳米杆型与脂质纳米玻璃。制备的抗G250 NTN的平均粒径为446nm。免疫荧光证实，抗G250纳米胚层均匀地分布在纳米泡的表面上。体外实验表明，抗G250 NTNs分别特异性地与G250阳性786-O细胞和HeLa细胞结合，具有88.13％+/- 4.37％和71.8％+/- 5.7％，它们没有结合到G250阴性Achn细胞。与空白纳米泡相比，抗G250 NTN可显着增强由786-O细胞和HeLa细胞产生的异种移植肿瘤的超声成像，而来自AchN细胞产生的异种移植肿瘤并不重要。肿瘤组织切片的免疫荧光证实，抗G250 NTN可以通过肿瘤血管进入组织空间并具体结合肿瘤细胞。总之，抗G250纳米型官能化靶向纳米博格尔可以特异性结合G250阳性RCC细胞，并增强G250阳性RCC异种移植物的超声成像。该研究对肾脏肿瘤的诊断和鉴别诊断具有高潜力的临床应用价值。

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