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Labeling-free detection of ECD-HER2 protein using aptamer-based nano-plasmonic sensor

机译：使用基于Aptamer的纳米等离子体传感器无标记检测ECD-HER2蛋白

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A gold nanoparticle-based localized surface plasmon resonance substrate has been developed as nano-sensors for various bio-applications. However, reproducible and robust sensing substrates anchored gold nanoparticles has not yet been explored. In this study, dopamine-coated gold nanorods (DGNRs) were prepared and immobilized onto the micro-grooving PDMS substrates (mgPDMS). Subsequently, HER2-specific aptamers were conjugated with DGNR/mgPDMS for ECD-HER2 detection. By screening of the optimal concentration of DGNR and aptamers, the effective HER2-specific aptasensor was built up. In particular, the real-time binding assay for the evaluation of limit-of-detection (<5 ng ml(-1)) was conducted. Furthermore, the binding kinetics for ECD-HER2 was investigated under the biological fluid using a rat serum. Our HER2-specific aptasensor demonstrated the effective sensitivity and selectivity for ECD-HER2.
机译：基于金纳米粒子的局部表面等离子体共振基板被开发为各种生物应用的纳米传感器。 然而，尚未探索锚定金纳米颗粒的可重复和稳健的感测基板。 在该研究中，制备多巴胺涂覆的金纳米棒（DGNR）并将其固定在微槽PDMS基板上（MGPDMS）上。 随后，将HER2特异性适体与DGNR / MGPDMS缀合，用于ECD-HER2检测。 通过筛选DGNR和适体的最佳浓度，建立了有效的HER2特异性APTASENOR。 特别地，对检测限制（<5ng ml（-1））进行评估的实时结合测定。 此外，使用大鼠血清在生物流体下研究了ECD-HER2的结合动力学。 我们的HER2特定的APTASENSOR展示了ECD-HER2的有效敏感性和选择性。

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