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Synthesis, characterization and applications of maghemite beads functionalized with rabbit antibodies

机译：用兔抗体官能化磁性珠珠的合成，表征及应用

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摘要

Magnetic nanoparticles (NPs) have attracted great attention owing to their applications in the biomedical field. In the present work, maghemite (gamma Fe2O3) NPs of 6.5 nm were prepared using a sonochemical method and used to prepare magnetic beads through silanization with 3-aminopropyltrimethoxysilane (APTS). Subsequently, amino groups in the resulting APTS-gamma Fe2O3 beads were converted to carboxylic acid (CARB-gamma Fe2O3) through the succinic anhydride reaction, as confirmed by transmission electron microscopy (TEM), Fourier transform infrared spectroscopy and dynamic light scattering (DLS) measurements. The size of these beads was measured as 12 nm and their hydrodynamic diameter as 490 nm, using TEM analysis and DLS, respectively. The CARB-gamma Fe2O3 beads were further functionalized by immobilizing rabbit antibodies on their surfaces; the immobilization was confirmed by flow cytometry and ionic strength. The samples were further characterized by Mossbauer spectroscopy and DC magnetization measurements. Studies on magnetic relaxivities showed that magnetic beads present great potential for application in MR imaging.
机译：由于它们在生物医学领域的应用，磁性纳米颗粒（NPS）引起了极大的关注。在本作工作中，使用一个SONOPEMICAL方法制备磁性镁膜（GAMMA FE2O3）NPS 6.5nm，并用3-氨基丙基三甲氧基硅烷（APTS）制备磁珠。随后，通过琥珀酸酐反应将所得APTS-Gamma Fe 2 O 3珠粒中的氨基转化为羧酸（Carb-Gamma Fe2O3），通过透射电子显微镜（TEM），傅里叶变换红外光谱和动态光散射（DLS）证实。测量。使用TEM分析和DLS测量这些珠子的尺寸和它们的流体动力直径为490nm。通过将兔抗体固定在其表面上的兔抗体进一步官能化CARB-Gamma Fe 2 O 3珠子;通过流式细胞术和离子强度证实固定化。该样品进一步特征，其特征在于Mossbauer光谱和直流磁化测量。关于磁性松弛的研究表明，磁珠在MR成像中存在巨大应用。

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  • 来源
    《Nanotechnology》 |2018年第36期|共10页
  • 作者

    Rodriguez A. F. R.; Rocha C. O.; Piazza R. D.; dos Santos C. C.; Morales M. A.; Faria F. S. E. D. V.; Iqbal M. Zubair; Barbosa L.; Chaves Y. O.; Mariuba L. A.; Jafelicci M. Jr.; Marques R. F. C.;

  • 作者单位

    Univ Fed Acre Lab Nanobiotechnol BR-69920900 Rio Branco AC Brazil;

    Sao Paulo State Univ UNESP Inst Chem Lab Magnet Mat &

    Colloids Caixa Postal 355 BR-14800900 Araraquara SP Brazil;

    Sao Paulo State Univ UNESP Inst Chem Lab Magnet Mat &

    Colloids Caixa Postal 355 BR-14800900 Araraquara SP Brazil;

    Sao Paulo State Univ UNESP Inst Chem Lab Magnet Mat &

    Colloids Caixa Postal 355 BR-14800900 Araraquara SP Brazil;

    Univ Fed Rio Grande do Norte Dept Theoret &

    Expt Phys BR-59078970 Natal RN Brazil;

    Univ Fed Acre Lab Nanobiotechnol BR-69920900 Rio Branco AC Brazil;

    Chinese Acad Sci Ningbo Inst Mat Technol &

    Engn Div Funct Mat &

    Nanodevices Beijing Peoples R China;

    Fundacao Oswaldo Cruz Inst Leonidas &

    Maria Deane BR-69057070 Adrianopolis AM Brazil;

    Fundacao Oswaldo Cruz Inst Leonidas &

    Maria Deane BR-69057070 Adrianopolis AM Brazil;

    Fundacao Oswaldo Cruz Inst Leonidas &

    Maria Deane BR-69057070 Adrianopolis AM Brazil;

    Sao Paulo State Univ UNESP Inst Chem Lab Magnet Mat &

    Colloids Caixa Postal 355 BR-14800900 Araraquara SP Brazil;

    Sao Paulo State Univ UNESP Inst Chem Lab Magnet Mat &

    Colloids Caixa Postal 355 BR-14800900 Araraquara SP Brazil;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 特种结构材料;
  • 关键词

    magnetic nanobeads; flow cytometry; MRI; mossbauer spectroscopy; XRD; rabbit antibody;

    机译：磁性纳米珠;流式细胞术;MRI;莫斯巴尔光谱;XRD;兔抗体;

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