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首页> 外文期刊>Nanotechnology >Label-free detection of live cancer cells and DNA hybridization using 3D multilayered plasmonic biosensor
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Label-free detection of live cancer cells and DNA hybridization using 3D multilayered plasmonic biosensor

机译：使用3D多层等离子传感器无标记检测活癌细胞和DNA杂交

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摘要

Three-dimensional (3D) multilayered plasmonic nanostructures consisting of Au nanosquares on top of SU-8 nanopillars, Au asymmetrical nanostructures in the middle, and Au asymmetrical nanoholes at the bottom were fabricated through reversal nanoimprint technology. Compared with two-dimensional and quasi-3D plasmonic nanostructures, the 3D multilayered plasmonic nanostructures showed higher electromagnetic field intensity, longer plasmon decay length and larger plasmon sensing area, which are desirable for highly sensitive localized surface plasmonic resonance biosensors. The sensitivity and resonance peak wavelength of the 3D multilayered plasmonic nanostructures could be adjusted by varying the offset between the top and bottom SU-8 nanopillars from 31% to 56%, and the highest sensitivity of 382 and 442 nm/refractive index unit were observed for resonance peaks at 581 and 805 nm, respectively. Live lung cancer A549 cells with a low concentration of 5 x 10(3) cells ml(-1) and a low sample volume of 2 mu l could be detected by the 3D multilayered plasmonic nanostructures integrated in a microfluidic system. The 3D plasmonic biosensors also had the advantages of detecting DNA hybridization by capturing the complementary target DNA in the low concentration range of 10(-14)-10(-7) M, and providing a large peak shift of 82 nm for capturing 10(-7) M complementary target DNA without additional signal amplification.
机译：由SU-8纳米粒子顶部的Au纳米颗粒组成的三维（3D）多层等离子体纳米结构，通过反转纳米压印技术制造底部的不对称纳米结构和底部的Au不对称纳米孔。与二维和准三维等离子体纳米结构相比，3D多层等离子体纳米结构显示出更高的电磁场强度，更长的等离子体衰减长度和较大的等离子体传感区域，这对于高敏感的局部表面等离子体共振生物传感器是期望的。通过从31％至56％的顶部和底部SU-8纳米粒子之间的偏移可以调节3D多层等离子体纳米结构的灵敏度和共振峰值波长，并且观察到382和442nm /折射率单元的最高灵敏度对于581和805nm的共振峰。通过集成在微流体系统中的3D多层等离子体纳米结构可以检测低浓度为5×10（3）个细胞m1（-1）的活肺癌A549细胞和2μl的低样品体积。 3D等离子体生物传感器还具有检测DNA杂交来检测DNA杂交，通过在10（-14）-10-10-10-10-10-10-10-10（-7）M中的互补靶DNA，并提供82nm的大峰值偏移，用于捕获10（ -7）M互补靶DNA没有额外的信号放大。

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