• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Nanotechnology >A CE-FL based method for real-time detection of in-capillary self-assembly of the nanoconjugates of polycysteine ligand and quantum dots
【24h】

A CE-FL based method for real-time detection of in-capillary self-assembly of the nanoconjugates of polycysteine ligand and quantum dots

机译：一种基于CE-FL的实时检测纳米缀合物和量子点纳米缀合物的毛细血管自组装的方法

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Small molecules with free thiol groups always show high binding affinity to quantum dots (QDs). However, it is still highly challenging to detect the binding capacity between thiol-containing molecules and QDs inside a capillary. To conquer this limitation, a capillary electrophoresis with fluorescence detection (CE-FL) based assay was proposed and established to investigate the binding capacity between QDs and a poly-thiolated peptide (ATTO 590-DDSSGGCCPGCC, ATTO-C4). Interestingly, the results showed that interval time had a great influence on QDs and ATTO-C4 self-assembly, which can be attributed to longer interval time benefitting the binding of QDs to ATTO-C4. The stability assays on ATTO-C4-QD assembly indicated that high concentration of imidazole or GSH had a high capability of competing with the bound ATTO-C4, evidenced by dramatically dropping of S-625/S-565 ratio from 0.78 to 0.30 or 0.29. Therefore, all these results above suggested that this novel CE-FL based detection assay could be successfully applied to the binding studies between QDs and thiol-containing biomolecules.
机译：具有游离硫醇基团的小分子总是对量子点（QDS）的高结合亲和力。然而，在毛细管内检测含硫醇的分子和QD的结合能力仍然具有强烈挑战性。为了征服这种限制，提出了一种基于荧光检测（CE-FL）的测定的毛细管电泳，并建立了研究QD和聚硫醇化肽（Atto 590-DDSSgCPGCC，Atto-C4）之间的结合能力。有趣的是，结果表明，间隔时间对QD和ATTO-C4自组装产生了很大影响，这可能归因于更长的间隔时间，使QDS与ATTO-C4的结合有益。 Atto-C4-QD组件上的稳定性测定表明，高浓度的咪唑或GSH具有高能力与结合的atto-C4竞争，通过大幅滴加S-625 / S-565比率为0.78至0.30或0.29 。因此，上述所有结果表明，该新型CE-FL基的检测测定可以成功地应用于QDS和含硫醇的生物分子之间的结合研究。

著录项

  • 来源
    《Nanotechnology》 |2018年第27期|共6页
  • 作者

    Wang Jianhao; Zhu Zhilan; Qiu Lin; Wang Jianpeng; Wang Xiang; Xiao Qicai; Xia Jiang; Liu Li; Liu Xiaoqian; Feng Wei; Wang Jinmei; Miao Peng; Gao Liqian;

  • 作者单位

    Changzhou Univ Sch Pharmaceut Engn &

    Life Sci Changzhou 213164 Jiangsu Peoples R China;

    Changzhou Univ Sch Pharmaceut Engn &

    Life Sci Changzhou 213164 Jiangsu Peoples R China;

    Changzhou Univ Sch Pharmaceut Engn &

    Life Sci Changzhou 213164 Jiangsu Peoples R China;

    Changzhou Univ Sch Pharmaceut Engn &

    Life Sci Changzhou 213164 Jiangsu Peoples R China;

    Nanjing Med Univ Affiliated Changzhou Peoples Hosp 2 Changzhou 213164 Jiangsu Peoples R China;

    SYSU Sch Pharmaceut Sci Shenzhen Guangzhou 510006 Guangdong Peoples R China;

    Chinese Univ Hong Kong Dept Chem Shatin Hong Kong Peoples R China;

    Changzhou Univ Sch Pharmaceut Engn &

    Life Sci Changzhou 213164 Jiangsu Peoples R China;

    Changzhou Univ Sch Pharmaceut Engn &

    Life Sci Changzhou 213164 Jiangsu Peoples R China;

    SYSU Sch Pharmaceut Sci Shenzhen Guangzhou 510006 Guangdong Peoples R China;

    SYSU Sch Pharmaceut Sci Shenzhen Guangzhou 510006 Guangdong Peoples R China;

    Chinese Acad Sci Suzhou Inst Biomed Engn &

    Technol Suzhou 215163 Peoples R China;

    SYSU Sch Pharmaceut Sci Shenzhen Guangzhou 510006 Guangdong Peoples R China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 特种结构材料;
  • 关键词

    capillary electrophoresis; peptide; quantum dots; self-assembly; Forster resonance energy transfer;

    机译：毛细管电泳;肽;量子点;自组装;福斯特共鸣能量转移;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号