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DNA nanostructure-based fluorescence thermometer with silver nanoclusters

机译：基于DNA纳米结构的荧光体温计与银纳米团簇

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摘要

DNA nanostructure-based fluorescence thermometers were fabricated by linking fluorescent silver nanoclusters (AgNCs) and guanine-rich (G-rich) DNA chains via a thermally sensitive DNA stem-loop at terminals 5' and 3'. Variations of temperature alter the distance between the AgNCs and G-rich DNA chain, affecting the interaction between them. As a result, the intensity of fluorescence emission from the AgNCs at 636 nm can be sensitively modulated. It was found that the intensity of such red emission is more temperature sensitive than the equivalent green emission at 543 nm; sensitivity of -3.6% / degrees C was achieved. Through variation of the melting temperature of the DNA stem-loop, the response temperature range of the thermometers could be readily adjusted. Novel DNA nanostructure-based fluorescence thermometers as described in this work are anticipated to be able to measure the temperature of biological systems at small scales-even a single cell.
机译：通过将荧光银纳米团簇（AGNCS）和富含富含的（G-Rich）的DNA链连接在端子5'和3'处通过热敏DNA茎环连接来制造基于DNA纳米结构的荧光温度计。 温度的变化改变了AgNC和富含G的DNA链之间的距离，影响它们之间的相互作用。 结果，可以敏感地调节来自636nm的AGNCs的荧光发射强度。 结果发现，这种红色发射的强度比543nm的等同绿色发射更敏感; 实现-3.6％/℃的敏感性。 通过DNA旋转环的熔化温度的变化，可以容易地调节温度计的响应温度范围。 本工作中描述的新型DNA纳米结构的荧光温度计预计将能够测量小鳞片的生物系统的温度 - 甚至是单个细胞。

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  • 来源
    《Nanotechnology》 |2018年第29期|共7页
  • 作者

    Bu Congcong; Mu Lixuan; Cao Xingxing; Chen Min; She Guangwei; Shi Wensheng;

  • 作者单位

    Chinese Acad Sci Tech Inst Phys &

    Chem Key Lab Photochem Convers &

    Optoelect Mat Beijing 100190 Peoples R China;

    Chinese Acad Sci Tech Inst Phys &

    Chem Key Lab Photochem Convers &

    Optoelect Mat Beijing 100190 Peoples R China;

    Chinese Acad Sci Tech Inst Phys &

    Chem Key Lab Photochem Convers &

    Optoelect Mat Beijing 100190 Peoples R China;

    Chinese Acad Sci Tech Inst Phys &

    Chem Key Lab Photochem Convers &

    Optoelect Mat Beijing 100190 Peoples R China;

    Chinese Acad Sci Tech Inst Phys &

    Chem Key Lab Photochem Convers &

    Optoelect Mat Beijing 100190 Peoples R China;

    Chinese Acad Sci Tech Inst Phys &

    Chem Key Lab Photochem Convers &

    Optoelect Mat Beijing 100190 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 特种结构材料;
  • 关键词

    thermometer; fluorescence; Ag nanoclusters; DNA stem-loop;

    机译：温度计;荧光;Ag纳米能器;DNA茎环;

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