• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Nanotechnology >Molecular beacon anchored onto a graphene oxide substrate
【24h】

Molecular beacon anchored onto a graphene oxide substrate

机译：将分子信标锚定到石墨烯氧化物衬底上

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In this article, we report a graphene oxide-based nanosensor incorporating semiconductor quantum dots linked to DNA-aptamers that functions as a 'turn-off' fluorescent nanosensor for detection of low concentrations of analytes. A specific demonstration of this turn-off aptasensor is presented for the case of the detection of mercury (II) ions. In this system, ensembles of aptamer-based quantum-dot sensors are anchored onto graphene oxide (GO) flakes which provide a platform for analyte detection in the vicinity of GO. Herein, the operation of this ensemble-based nanosensor is demonstrated for mercury ions, which upon addition of mercury, quenching of the emission intensity from the quantum dots is observed due to resonance energy transfer between quantum dots and the gold nanoparticle connected via a mercury target aptamer. A key result is that the usually dominant effect of quenching of the quantum dot due to close proximity to the GO can be reduced to negligible levels by using a linker molecule in conjunctions with the aptamer-based nanosensor. The effect of ionic concentration of the background matrix on the emission intensity was also investigated. The sensor system is found to be highly selective towards mercury and exhibits a linear behavior (r(2) > 0.99) in the nanomolar concentration range. The detection limit of the sensor towards mercury with no GO present was found to be 16.5 nM. With GO attached to molecular beacon via 14 base, 35 base, and 51 base long linker DNA, the detection limit was found to be 38.4 nM, 9.45 nM, and 11.38 nM; respectively.
机译：在本文中，我们报告了一种掺入与DNA - 适体连接的半导体量子点的基于石墨烯的纳米传感器，其用作“关闭”荧光纳米传感器，用于检测低浓度的分析物。为汞（II）离子检测的情况提出了该关断的Aptasensor的具体示范。在该系统中，基于Aptamer的量子点传感器的集合锚固到石墨烯氧化物（GO）薄片上，该薄片提供了在Go附近进行分析物检测的平台。在此，对汞离子的汞离子对该基于组合的纳米传感器的操作进行了说明，这在加入汞后，由于通过汞靶向的量子点和金纳米粒子之间的谐振能量传递，观察到来自量子点的发光强度的淬火适体。一个关键的结果是，通过使用基于Aptamer的纳米传感器的连杆，可以减少由于近距离的近距离近距离近距离的量子点猝灭的主要效果可以减少到可忽略的水平。还研究了背景基质离子浓度对发射强度的影响。发现传感器系统对汞具有高度选择性，并且在纳米摩尔浓度范围内显示出线性行为（R（2）> 0.99）。发现传感器对没有目前的汞的检测限为16.5nm。通过14碱基，35碱基和51个碱基长接头DNA连接到分子信标，发现检测限为38.4nm，9.45nm和11.38nm;分别。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号