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首页> 外文期刊>Nature reviews Cancer >Avicularin ameliorates human hepatocellular carcinoma via the regulation of NF-kappa B/COX-2/PPAR-gamma activities
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Avicularin ameliorates human hepatocellular carcinoma via the regulation of NF-kappa B/COX-2/PPAR-gamma activities

机译：Avicularin通过调节NF-Kappa B / Cox-2 / PPAR-Gamma活性来改善人肝细胞癌

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摘要

Hepatocellular carcinoma (HCC) has become a global public health problem. Therefore, the development of novel and effective therapeutic agents for the treatment of HCC is considered an emergency. Avicularin, a bio-active flavonoid from plants, has been reported to exhibit diverse pharmacological properties. The aim of the present study was to investigate the role of avicularin in HCC and the underlying mechanism of action. Huh7 cells were treated with avicularin in a concentration-dependent manner, and the cell proliferation was examined using a 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay kit. The cell migration and invasion abilities were detected using wounding-healing assays and Transwell assays. Flow cytometric analysis was performed to investigate the cell cycle distribution and cell apoptosis. The activity of nuclear factor (NF)-kappa B (p65), cyclooxygenase-2 (COX-2) and peroxisome proliferator-activated receptor gamma (PPAR-gamma) were measured by reverse transcription-quantitative polymerase chain reaction and western blot analyses, respectively. The results indicated that avicularin treatment markedly decreased cell proliferation concentration-dependently in HCC, and inhibited cell migration and invasion in Huh7 cells. It was also found that the treatment of avicularin markedly inhibited the G0/G1-phase cells and decreased the accumulation of S-phase cells in the cell cycle and induced cell apoptosis. In addition, it was confirmed that the anticancer efficacy of avicularin in HCC was dependent on the regulation of NF-kappa B (p65), COX-2 and PPAR-gamma activities. In conclusion, the findings suggested that avicularin serves an antineoplastic role in HCC and may provide a potential therapeutic strategy for the treatment of HCC.
机译：肝细胞癌（HCC）已成为全球公共卫生问题。因此，用于治疗HCC的新型和有效治疗剂的发展被认为是紧急情况。据报道，来自植物的生物活性黄酮类生物活性黄酮，表现出不同的药理学特性。本研究的目的是探讨Avicularin在HCC中的作用及其行动潜在机制。 Huh7细胞以浓度依赖性的方式用植青蛋白处理，使用3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑溴铵测定试剂盒检查细胞增殖。使用伤口愈合测定和Transwell测定检测细胞迁移和侵袭能力。进行流式细胞术分析以研究细胞周期分布和细胞凋亡。通过逆转录定量聚合酶链反应和Western印迹分析测量核因子（NF）-Kappa B（P65），环氧氧酶-2（COX-2）和过氧化物体增殖物激活受体γ（PPAR-Gamma）的活性。分别。结果表明，抗植物治疗在HCC中依赖性显着降低了细胞增殖，并抑制了HUH7细胞中的细胞迁移和侵袭。还发现，抗植物的治疗明显抑制了G0 / G1相细胞，并降低了细胞周期中S相细胞的积累和诱导细胞凋亡。此外，证实HCC中抗胰素的抗癌疗效取决于NF-Kappa（P65），COX-2和PPAR-Gamma活性的调节。总之，该研究结果表明，Avicularin在HCC中用于抗肿瘤作用，可为治疗HCC提供潜在的治疗策略。

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