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首页> 外文期刊>Nature reviews Cancer >Streamlined Development of Targeted Mass Spectrometry-Based Method Combining Scout-MRM and a Web-Based Tool Indexed with Scout Peptides
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Streamlined Development of Targeted Mass Spectrometry-Based Method Combining Scout-MRM and a Web-Based Tool Indexed with Scout Peptides

机译：基于侦察-MRM的基于靶标质谱的方法的简化开发与侦察肽分析了侦察肽的基于Web的工具

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MS-based targeted proteomics is a relevant technology for sensitive and robust relative or absolute quantification of proteins biomarker candidates in complex human biofluids or tissue extracts. Performing a multiplex assay imposes time scheduling of peptide monitoring only around their expected retention time that needs to be defined with synthetic peptide. Time-scheduled monitoring is clearly a constraint that precludes from straightforward assay transfer between biological matrices or distinct experimental setup. Any unexpected retention time (RT) shift challenges assay robustness and its implementation for large-scale analysis. Recently, Scout-multiple reaction monitoring that fully releases multiplexed targeted acquisition from RT scheduling by successively monitoring complex transition groups triggered with sentinel molecules called Scout has been introduced. It is herein documented how Peptide Selector database and tool streamlines the building of a multiplexed method thanks to RT indexation relative to Scout peptides. This case study deals with surrogate peptides of biomarker candidates related to drug-induced liver and vascular injury, running such on-line built method (eight Scouts triggering the monitoring of a total of 692 transitions) enables 100% recovery of a panel of 93 spiked-in heavy labeled standards, despite significant RT shifts between serum, plasma, or urine. This result illustrates the simplicity of automatically building and deploying robust proteomics targeted assay.
机译：基于MS的靶向蛋白质组学是一种用于复杂人生物流体或组织提取物中蛋白质生物标志物候选蛋白质生物标志物候选的敏感和鲁棒的相关技术的相关技术。进行多重测定施加仅围绕其预期保留时间的肽监测的时间调度，需要用合成肽定义。时间预定的监测显然是一种限制，其缺乏生物基质或不同实验设置之间的直接测定转移。任何意外的保留时间（RT）转移挑战策划稳健性及其对大规模分析的实施。最近，通过介绍了通过连续监测名为Scout触发的复合转换组来完全释放从RT调度的多路复用目标采集的侦察多重反应监测。这里记录了肽选择器数据库和工具如何简化复用方法的建筑物，得益于RT指数相对于侦察肽。本案例研究涉及与药物诱导的肝脏和血管损伤相关的生物标志物候选的替代肽，这种在线建造方法（八侦察触发总共692道的监测）使得能够100％回收93面板飙升 - 尽管血清，血浆或尿液之间存在显着的RT偏移，但重标标准。该结果说明了自动构建和部署鲁棒蛋白质组学的靶向测定的简单性。

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