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首页> 外文期刊>Biochemical Engineering Journal >Biodiesel production catalyzed by Rhizomucor miehei lipase-displaying Pichia pastoris whole cells in an isooctane system
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Biodiesel production catalyzed by Rhizomucor miehei lipase-displaying Pichia pastoris whole cells in an isooctane system

机译：异辛烷系统中展示米氏根霉的脂肪酶催化巴斯德毕赤酵母全细胞催化的生物柴油生产

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摘要

A recombinant Pichia pastoris displaying Rhizomucor miehei Lipase (RML) on the cell surface was shown to be an efficient biocatalyst in biodiesel production from soybean oil. Various reaction parameters affecting the transesterification catalyzed by RML-displaying P. pastoris whole cells were studied. The optimal reaction conditions were: temperature 55 °C, water activity 0.66, the ratio of isooctane to oil 2:1 (v/v) and the whole-cell biocatalyst 40% (w/w, soybean oil). To reduce the inactivation of displaying lipases by methanol, each 1.5 molar equivalent of methanol (mol/mol, soybean oil) were added in a three-step procedure at the reaction time of 0 h, 24 h and 48 h, respectively. Under these optimized conditions, a methyl esters (ME) yield reached 83.14% after 72 h, which significantly enhanced the ME yield catalyzed by the displaying lipase reported previously. The RML-displaying P. pastoris whole-cell biocatalyst exhibited quite a good operational stability with more than 80.46% of its original activity remained even after 10 batches reaction as well as storage stability. Thus, it showed promising potential in biodiesel production.
机译：重组巴斯德毕赤酵母在细胞表面显示出根瘤菌脂肪酶（RML）表现出是从大豆油生产生物柴油的有效生物催化剂。研究了影响展示RML的巴斯德毕赤酵母全细胞催化酯交换反应的各种反应参数。最佳反应条件为：温度55°C，水活度为0.66，异辛烷与油的比例为2：1（v / v），全细胞生物催化剂为40％（w / w，大豆油）。为了减少甲醇对展示脂酶的失活，在三步法中分别在0h，24h和48h的反应时间加入1.5摩尔当量的甲醇（mol / mol，豆油）。在这些优化的条件下，甲基酯（ME）的收率在72 h后达到83.14％，这大大提高了先前报道的展示脂酶催化的ME收率。展示RML的巴斯德毕赤酵母全细胞生物催化剂表现出相当好的操作稳定性，即使经过10批反应，其原始活性仍保持超过80.46％的稳定性，并具有储存稳定性。因此，它在生物柴油生产中显示出令人鼓舞的潜力。

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