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首页> 外文期刊>Life sciences >Protein kinases C isozymes are differentially expressed in human breast carcinomas
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Protein kinases C isozymes are differentially expressed in human breast carcinomas

机译：蛋白激酶C同工酶在人乳腺癌中差异表达

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Aims: The protein kinase C (PKC) family of enzymes has been implicated in cellular proliferation, differentiation, and apoptosis. However, the distribution of specific PKC isoforms with varying functions in normal and malignant human tissues remains to be determined. The objective of this study was to investigate the expression of certain PKC isoforms (alpha, betaI, betaII, epsilon) in human breast cancer specimens relative to adjacent uninvolved tissue (n=24) and in the normal breast tissue obtained from patients undergoing reduction mammoplasty (n=12).Main methods: Western blot analysis using PKC isoform specific antibodies was performed on tissue extracts from breast tumors, adjacent uninvolved tissues, and reduction mammoplasty tissues.Key findings: Mean levels of cytosolic and membrane PKC-alpha, PKC-betaI, and PKC-betaII were significantly higher in the cancer specimens than in the adjacent uninvolved breast tissues (Wilcoxon signed-ranks test; P<0.05 for each, after adjustment for multiple comparisons). There was a notably higher mean level of membrane PKC-betaII isozyme in Her-2 positive and in poorly differentiated tumors. No significant differences were observed when normal tissue adjacent to tumor was compared to breast tissue obtained from reduction mammoplasty specimens. Significance: Higher level of PKC-alpha, PKC-betaI, and PKC-betaII in cancer specimens and higher level of PKC-betaII in Her-2 positive tumors require further exploration of the intracellular pathways involving PKC-alpha and -beta isoforms in breast cancer because both could be specific targets for the development of new therapies and for the prevention and treatment of this disease.
机译：目的：蛋白激酶C（PKC）酶系列涉及细胞增殖，分化和细胞凋亡。然而，在正常和恶性人体组织中具有不同功能的特异性PKC同种型的分布仍有待确定。本研究的目的是探讨人乳腺癌标本中某些PKC同种型（Alpha，Betai，Betaii，Epsilon）的表达相对于相邻的未植入组织（n = 24），并在从经历减少乳腺成形术的患者获得的正常乳房组织中（n = 12）.MAIN方法：使用PKC同种型特异性抗体的Western印迹分析对来自乳腺肿瘤，邻近的未植入组织和还原乳腺成形术组织的组织提取物进行.Key表现：细胞溶质和膜PKC-α的平均水平，PKC- β癌和PKC-Betaii在癌症标本中显着高于相邻的未植入的乳腺组织（Wilcoxon签名 - 排名试验;每次P <0.05，调整多重比较后）。在HER-2阳性和肿瘤差的肿瘤中存在显着高的膜PKC-BETAII同工酶。当肿瘤邻近的正常组织与从减少乳腺成形术样品获得的乳腺组织进行比较时，没有观察到显着差异。意义：癌症标本中的高水平PKC-α，PKC-Betai和PKC-Betaii在HER-2阳性肿瘤中的PKC-Betaii水平需要进一步探索乳腺癌中PKC-α和-Beta同种型的细胞内途径癌症，因为两者都可能是开发新疗法和预防和治疗这种疾病的特定目标。

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