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首页> 外文期刊>Life sciences >Presence of ChAT mRNA and a very marked α7nAChR immunoreaction in the synovial lining layer of the knee joint.
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Presence of ChAT mRNA and a very marked α7nAChR immunoreaction in the synovial lining layer of the knee joint.

机译：在膝关节的滑膜衬里层中存在聊天mRNA和非常明显的α7NACHR免疫反应。

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摘要

The aim was to examine if there is evidence of acetylcholine (ACh) production within the synovial lining layer and to examine the pattern of α7nAChR expression in the layer. This layer is of relevance clinically as it becomes thickened in response to both rheumatoid arthritis (RA) and osteoarthritis (OA) and as it has been shown to produce proteases that are involved in the cartilage destruction.Synovial tissue specimens from the knee joint of patients with RA and OA undergoing prosthetic surgery were examined. In situ hybridization and immunohistochemistry were used for the evaluation of ChAT reaction patterns. Immunohistochemistry was utilized for demonstration of activity of α7nAChR.There were ChAT mRNA reactions in the synovial lining layer of both patient categories. On the other hand, no ChAT immunoreactions were detected in the layer. There was a very marked α7nAChR immunoreaction.There is a potential for ACh production within the synovial lining layer as there are ChAT mRNA reactions. However, the level of ACh production is apparently very low. It is thus possible that there is a down-regulation of ACh production but an apparent upregulation in expression level of α7nAChR. Based on the knowledge that the non-neuronal cholinergic system can have anti-inflammatory effects, the low level of ACh production in the synovial lining layer can be a drawback for the arthritic joints.
机译：目的是检查是否存在乙酰胆碱（ACH）在滑膜层内产生的证据，并检查层中α7nAChr表达的图案。该层在临床上具有相关性，因为响应类风湿性关节炎（RA）和骨关节炎（OA）而变化，并且由于已经显示出涉及软骨破坏的蛋白酶。来自患者膝关节的同步组织标本随着RA和OA进行了接​​受假肢手术。原位杂交和免疫组织化学用于评估聊天反应模式。免疫组织化学用于证明α7NACHR的活性。在两个患者类别的滑膜层中聊天mRNA反应。另一方面，在层中没有检测到聊天免疫液。有一个非常明显的α7nachr免疫反应。在滑膜衬里层内的ACH生产可能是聊天mRNA反应。然而，ACH生产水平显然非常低。因此，可以在α7NACHR的表达水平下存在对ACH生产的下调，但是表达水平的表现上调。基于非神经元胆碱能系统可以具有抗炎作用的知识，滑膜衬层中的低水平的ACH生产可以是关节炎关节的缺点。

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  • 来源
    《Life sciences》 |2012年第22期|共5页
  • 作者

    Sture Forsgren;

  • 作者单位

    Department of Integrative Medical Biology Anatomy Section Ume? University SE-901 87 Ume? Sweden.;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 医药、卫生;
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