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首页> 外文期刊>Life sciences >Presence of ChAT mRNA and a very marked α7nAChR immunoreaction in the synovial lining layer of the knee joint.
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Presence of ChAT mRNA and a very marked α7nAChR immunoreaction in the synovial lining layer of the knee joint.

机译：膝关节滑膜衬里层中存在ChAT mRNA和非常明显的α7nAChR免疫反应。

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摘要

The aim was to examine if there is evidence of acetylcholine (ACh) production within the synovial lining layer and to examine the pattern of α7nAChR expression in the layer. This layer is of relevance clinically as it becomes thickened in response to both rheumatoid arthritis (RA) and osteoarthritis (OA) and as it has been shown to produce proteases that are involved in the cartilage destruction.Synovial tissue specimens from the knee joint of patients with RA and OA undergoing prosthetic surgery were examined. In situ hybridization and immunohistochemistry were used for the evaluation of ChAT reaction patterns. Immunohistochemistry was utilized for demonstration of activity of α7nAChR.There were ChAT mRNA reactions in the synovial lining layer of both patient categories. On the other hand, no ChAT immunoreactions were detected in the layer. There was a very marked α7nAChR immunoreaction.There is a potential for ACh production within the synovial lining layer as there are ChAT mRNA reactions. However, the level of ACh production is apparently very low. It is thus possible that there is a down-regulation of ACh production but an apparent upregulation in expression level of α7nAChR. Based on the knowledge that the non-neuronal cholinergic system can have anti-inflammatory effects, the low level of ACh production in the synovial lining layer can be a drawback for the arthritic joints.
机译：目的是检查滑膜衬里层中是否有乙酰胆碱（ACh）产生的证据，并检查该层中α7nAChR表达的模式。该层在临床上具有相关性，因为它对类风湿性关节炎（RA）和骨关节炎（OA）都有反应而变厚，并且已经显示出可产生与软骨破坏有关的蛋白酶。患者膝关节的滑膜组织标本对RA和OA进行假体手术的患者进行了检查。原位杂交和免疫组化用于ChAT反应模式的评估。免疫组织化学法用于证明α7nAChR的活性。两种患者的滑膜衬里层都有ChAT mRNA反应。另一方面，在该层中未检测到ChAT免疫反应。出现了非常明显的α7nAChR免疫反应，由于ChAT mRNA反应，在滑膜衬里层内可能产生ACh。但是，乙酰胆碱的生产水平显然很低。因此，ACh产生可能下调，而α7nAChR的表达水平却明显上调。基于非神经胆碱能系统可以具有抗炎作用的认识，滑膜衬里层中低水平的ACh产生可能是关节炎关节的缺点。

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