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首页> 外文期刊>Life sciences >Both neuronal and non-neuronal acetylcholine take part in non-quantal acetylcholine release in the rat atrium.
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Both neuronal and non-neuronal acetylcholine take part in non-quantal acetylcholine release in the rat atrium.

机译：神经元和非神经元乙酰胆碱均参与大鼠中胞内的非量子乙酰胆碱释放。

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摘要

In mammalian myocardium acetylcholine (ACh), neurotransmitter which strikingly affects the cardiomyocytes, can be released from the neurons both via quantal (vesicular) and nonquantal (non-vesicular) mechanism of secretion. Non-quantal release is continuous, independent on vagus activity and provides accumulation of ACh in myocardium in the presence of acetylcholinesterase (AChE) inhibitors. The aim of the present study was to determine the source of non-quantal ACh in isolated atrial myocardium of adult and newborn rats.Standard microelectrode technique was used to determine the cholinergic changes of electrical activity under the action of AChE inhibitor paraoxon, which correlates with the intensity of nonquantal ACh release.In adult rats selective inhibitor of neuronal choline uptake system hemicholinium III (10(-5) M) decreased all effects of paraoxon (5 × 10(-6) M) more than twofold. Inhibitor of polyspecific 3 organic cation transporters corticosterone (10(-4) M) also significantly decreased effects of paraoxon in adult rats, indicating that non-neuronal ACh, which is synthesized by cardiomyocytes, takes part in accumulation of ACh in the myocardium. When hemicholinium III and corticosterone were applied together, paraoxon effects in adult atrial myocardium were suppressed almost completely. In newborn rats cardiomyocytes do not excrete ACh. In accordance with this fact hemicholinium III completely abolished effects of paraoxon in newborn myocardium, while corticosterone was ineffective. Thus, non-quantal ACh is released both from cholinergic nerves and cardiomyocytes in adult rat myocardium, while it has exclusively neuronal nature in newborns.The study demonstrates dual neuronal and non-neuronal nature of non-quantal ACh in the heart.
机译：在哺乳动物心肌乙酰胆碱（ACH）中，突出地影响心肌细胞的神经递质，可以通过量子（囊状）和分泌非夸构（非囊肿）机制从神经元释放。非量子释放是连续的，独立于迷走法，在乙酰胆碱酯酶（疼痛）抑制剂存在下提供心肌的ACH积累。本研究的目的是确定成人和新生大鼠的孤立心房心肌中的非量子疼痛来源。标准的微电极技术用于确定ACHE抑制剂拮抗剂作用下的电活性的胆碱能变化，与...相关非致脉冲释放的强度。成年大鼠神经元胆碱摄取系统的选择性抑制剂血清腺素III（10（-5）m）降低了副索（5×10（-6）m）的所有效果。多特异性3有机阳离子转运蛋白皮质酮（10（-4）M）的抑制剂也显着降低了成年大鼠的副植物的影响，表明由心肌细胞合成的非神经元ACH参与了心肌中ACH的积累。当血清素III和皮质酮一起应用时，几乎完全抑制了成年心房心肌中的律福音效应。在新生大鼠中，心肌细胞不会排除疼痛。根据这一事实，血清腺素III完全消除了律植物在新生儿心肌中的作用，而皮质酮无效。因此，非量子ACH从成年大鼠心肌中的胆碱能神经和心肌细胞释放出来，而新生儿在其中具有神经性的性质。该研究表明了心脏中非衡量ACH的双神经元和非神经元性质。

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