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首页> 外文期刊>Life sciences >Anti-inflammatory effect of transduced PEP-1-Cyclophilin A in Raw 264.7 cells and 12-O-tetradecanoylphorbol-13-acetate-induced mice.
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Anti-inflammatory effect of transduced PEP-1-Cyclophilin A in Raw 264.7 cells and 12-O-tetradecanoylphorbol-13-acetate-induced mice.

机译：转导PEP-1-环旋蛋白A在原料264.7细胞中的抗炎作用和12-四癸酰卟啉-13-乙酸酯诱导的小鼠。

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摘要

AIMS: Cyclophilin A (CypA) is an immunophilin that acts as a receptor for the immunosuppressant drug cyclosporine A (CsA). CypA has emerged as a potential drug target for several inflammatory diseases, although the details of its mechanism are unclear. We examined the protective effects of CypA on inflammation in Raw 264.7 cells and animal models. MAIN METHODS: A human CypA gene was fused with a protein transduction domain, PEP-1 peptide, to construct a cell permeable PEP-1-CypA protein. The protein expression level of cyclooxygenase-2 (COX-2) and cytokines was detected by Western blot, ELISA and mRNA level of COX-2 and cytokines were measured by RT-PCR. The nuclear factor-kappa B (NF-kB) and mitogen-activated protein kinase (MAPK) activation were analyzed by Western blot and electrophoretic mobility shift assay. Skin inflammation was detected with immunohistochemistry. KEY FINDINGS: Transduced PEP-1-CypA protein markedly inhibited lipopolysaccharide- and 12-O-tetradecanoyl phorbol-13-acetate-induced expression levels of COX-2 as well as pro-inflammatory cytokine levels in vitro and in vivo. Furthermore, transduced PEP-1-CypA protein resulted in a significant reduction in the activation of NF-kB and MAPK. SIGNIFICANCE: The results indicate that PEP-1-CypA inhibits inflammatory response cytokines and enzymes by blocking NF-kB and MAPK activation upon stimulation of inflammation in vitro and in vivo. PEP-1-CypA protein may potentially be used as a therapeutic agent against skin diseases-related inflammation.
机译：目的：细胞环素A（CYPA）是一种免疫蛋白，其作为免疫抑制药物环孢菌素A（CSA）的受体。 Cypa已成为几种炎症性疾病的潜在药物靶标，尽管其机制的细节尚不清楚。我们检查了CYPA对原始264.7细胞和动物模型中炎症的保护作用。主要方法：将人Cypa基因与蛋白质转导域，Pep-1肽融合，构建细胞可渗透的PEP-1-CYPA蛋白。通过蛋白质印迹检测环氧氧酶-2（COX-2）和细胞因子的蛋白质表达水平，通过RT-PCR测量COX-2和细胞因子的ELISA和mRNA水平。通过蛋白质印迹和电泳迁移率移位测定分析核因子-Kappa（NF-Kb）和丝裂剂活化的蛋白激酶（MAPK）活化。用免疫组织化学检测皮肤炎症。关键发现：转导PEP-1-CYPA蛋白显着抑制脂多糖和12- o-四癸酰易孔-13-乙酸酯诱导的COX-2的表达水平，以及体内和体内促炎细胞因子水平。此外，转导的PEP-1-CYPA蛋白导致NF-KB和MAPK活化的显着降低。意义：结果表明，通过在体外刺激炎症和体内刺激炎症时，Pep-1-Cypa通过阻断NF-KB和MAPK活化来抑制炎症反应细胞因子和酶。 PEP-1-CYPA蛋白可能被用作针对皮肤病相关炎症的治疗剂。

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