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首页> 外文期刊>RSC Advances >Computer-aided design of magnetic molecularly imprinted polymer nanoparticles for solid-phase extraction and determination of levetiracetam in human plasma
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Computer-aided design of magnetic molecularly imprinted polymer nanoparticles for solid-phase extraction and determination of levetiracetam in human plasma

机译：用于固相萃取的磁性分子印迹聚合物纳米粒子的计算机辅助设计和人血浆中Levetiracetam的测定

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摘要

Analytical methods should be accurate and specific to measure plasma drug concentration. Nevertheless, current sample preparation techniques suffer from limitations, including matrix interference and intensive sample preparation. In this study, a novel technique was proposed for the synthesis of a molecularly imprinted polymer (MIP) on magnetic Fe3O4 nanoparticles (NPs) with uniform core-shell structure. The Fe3O4@MIPs NPs were then applied to separate and enrich an antiepileptic drug, levetiracetam, from human plasma. A computational approach was developed to screen the functional monomers and polymerization solvents to provide a suitable design for the synthesized MIP. Different analysis techniques and re-binding experiments were performed to characterize the Fe3O4@MIP NPs, as well as to identify optimal conditions for the extraction process. Adsorption isotherms were best fitted to the Langmuir model and adsorption kinetics were modeled with pseudo-second-order kinetics. The Fe3O4@MIP NPs showed reasonable adsorption capacity and improved imprinting efficiency. A validated colorimetric assay was introduced as a comparable method to a validated HPLC assay for the quantitation of levetiracetam in plasma in the range of 10-80 g mL(-1) after extraction. The results from the HPLC and colorimetric assays showed good precision (between 1.08% and 9.87%) and recoveries (between 94% and 106%) using the Fe3O4@MIP NPs. The limit of detection and limit of quantification were estimated to be 2.58 g mL(-1) and 7.81 g mL(-1), respectively for HPLC assay and 2.32 g mL(-1) and 7.02 g mL(-1), respectively for colorimetric assay. It is believed that synthesized Fe3O4@MIP NPs as a sample clean-up technique combined with the proposed assays can be used for determination of levetiracetam in plasma.
机译：分析方法应准确且特异性地测量血浆药物浓度。然而，目前的样品制备技术遭受限制，包括基质干扰和密集的样品制备。在该研究中，提出了一种新的技术，用于用均匀的核壳结构合成磁Fe3O4纳米颗粒（NPS）的分子印迹聚合物（MIP）。然后施用Fe3O4 @ MIPS NPS以分离并丰富来自人血浆的抗癫痫药物Levetiracetam。开发了计算方法以筛选功能性单体和聚合溶剂，为合成的壁制成合适的设计。进行不同的分析技术和重新结合实验以表征Fe3O4 @ MIP NP，以及鉴定提取过程的最佳条件。吸附等温线最适合朗马尔模型，吸附动力学与伪二阶动力学进行建模。 FE3O4 @ MIP NPS显示合理的吸附容量和改善的印迹效率。将验证的比色测定作为与验证的HPLC测定的验证的HPLC测定引入了验证的HPLC测定，以在萃取后的血浆中的血浆中的左右乙酰胺（-1）的范围内。 HPLC和比色测定的结果显示使用Fe3O4 @ MIP NPS的良好精度（1.08％和9.87％），回收率（94％和106％之间）。分别估计检测极限和定量的限制分别为HPLC测定和2.32g mL（-1）和7.02g ml（-1）的2.58g ml（-1）和7.81g ml（-1）用于比色测定。据信，合成的Fe3O4 @ MIP NPS作为样品清洁技术与所提出的测定相结合，可用于测定血浆中的Levetiracetam。

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  • 来源
    《RSC Advances》 |2018年第26期|共13页
  • 作者

    Attallah Olivia A.; Al-Ghobashy Medhat A.; Ayoub Ahmed Taha; Tuszynski Jack Adam; Nebsen Marianne;

  • 作者单位

    Heliopolis Univ Pharmaceut Chem Dept Cairo Belbeis Desert Rd El Salam 11777 Cairo Governora Egypt;

    Cairo Univ Analyt Chem Dept Fac Pharm Cairo 11562 Egypt;

    Heliopolis Univ Pharmaceut Chem Dept Cairo Belbeis Desert Rd El Salam 11777 Cairo Governora Egypt;

    Univ Alberta Dept Phys Edmonton AB T6G 2E1 Canada;

    Cairo Univ Analyt Chem Dept Fac Pharm Cairo 11562 Egypt;

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  • 正文语种 eng
  • 中图分类 化学;
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