Defined covalent assembly of protein molecules on graphene using a genetically encoded photochemical reaction handle

Zaki Athraa J.; Hartley Andrew M.; Reddington Samuel C.; Thomas Suzanne K.; Watson Peter; Hayes Anthony; Moskalenko Andy V.; Craciun Monica F.; Macdonald J. Emyr; Jones D. Dafydd; Elliott Martin

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Defined covalent assembly of protein molecules on graphene using a genetically encoded photochemical reaction handle

机译：使用遗传编码的光化学反应手柄定义在石墨烯上的蛋白质分子的共价组装

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We have created modified protein variants by introducing a non-canonical amino acid p-azido-L-phenylalanine (azF) into defined positions for photochemically-induced covalent attachment to graphene. Attachment of GFP, TEM and cyt b(562) proteins was verified through a combination of atomic force and scanning tunnelling microscopy, resistance measurements, Raman data and fluorescence measurements. This method can in principle be extended to any protein which can be engineered in this way without adversely affecting its structural stability.

机译：通过将非规范氨基酸P-苯胺-1-苯丙氨酸（AZF）引入定义的位置，通过将非典型的氨基酸P-苯胺 - L-苯丙氨酸（AZF）引入光化学诱导的共价附着于石墨烯的定义位置来产生改性蛋白质变体。通过原子力和扫描隧道显微镜，电阻测量，拉曼数据和荧光测量，通过组合验证GFP，TEM和CYT B（562）蛋白的附着。本方法原则上可以延伸到任何可以以这种方式设计的蛋白质，而不会对其结构稳定性产生不利影响。

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来源
《RSC Advances》 |2018年第11期|共8页
作者
Zaki Athraa J.; Hartley Andrew M.; Reddington Samuel C.; Thomas Suzanne K.; Watson Peter; Hayes Anthony; Moskalenko Andy V.; Craciun Monica F.; Macdonald J. Emyr; Jones D. Dafydd; Elliott Martin;
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作者单位

Cardiff Univ Sch Phys &

Astron Cardiff CF24 3AA S Glam Wales;

Cardiff Univ Sch Biosci Cardiff CF10 3AX Wales;

Cardiff Univ Sch Biosci Cardiff CF10 3AX Wales;

Cardiff Univ Sch Phys &

Astron Cardiff CF24 3AA S Glam Wales;

Cardiff Univ Sch Biosci Cardiff CF10 3AX Wales;

Cardiff Univ Sch Biosci Cardiff CF10 3AX Wales;

Cardiff Univ Sch Phys &

Astron Cardiff CF24 3AA S Glam Wales;

Univ Exeter Ctr Graphene Sci Exeter EX4 4QF Devon England;

Cardiff Univ Sch Phys &

Astron Cardiff CF24 3AA S Glam Wales;

Cardiff Univ Sch Biosci Cardiff CF10 3AX Wales;

Cardiff Univ Sch Phys &

Astron Cardiff CF24 3AA S Glam Wales;

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正文语种 eng
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1. Defined covalent assembly of protein molecules on graphene using a genetically encoded photochemical reaction handle [J] . Zaki Athraa J., Hartley Andrew M., Reddington Samuel C., RSC Advances . 2018,第11期

机译：使用遗传编码的光化学反应手柄定义在石墨烯上的蛋白质分子的共价组装
2. Genetically encoded photochemical covalent crosslinking within the Hcp-1 self-assembling bacterial secretion machinery (vol 50, pg 641, 2018) [J] . Antonczak Alicja K., Milholland Kedric, Tippmann Eric M. Amino acids . 2019,第3期

机译：遗传编码在HCP-1自组装细菌分泌机械内的光化学共价交联（Vol 50，PG 641,2018）
3. Genetically encoded photochemical covalent crosslinking within the Hcp-1 self-assembling bacterial secretion machinery [J] . Antonczak Alicja K., Milholland Kedric, Tippmann Eric M. Amino acids . 2018,第5期

机译：HCP-1自组装细菌分泌机械内的遗传编码光化学共价交联
4. MULTI-STEP REACTIONS ON SILICA SURFACES FOR ANCHORING PROTEINS BY MEANS OF COVALENT LINKAGES [C] . Xue Sha, Chengjun Sun, Lynn S. Penn Annual meeting of the Adhesion Society . 2012

机译：价键连接在硅胶表面上的多步法固定蛋白
5. Covalent lipoprotein(a) assembly Characterization of oxidase activity responsible for catalyzing covalent lipoprotein(a) assembly [D] . Sledziecka, Anna Katarzyna. 2008

机译：共价脂蛋白（a）装配负责催化共价脂蛋白（a）装配的氧化酶活性的表征
6. Role of the H Protein in Assembly of the Photochemical Reaction Center and Intracytoplasmic Membrane in Rhodospirillum rubrum [O] . Yongjian S. Cheng, Christine A. Brantner, Alexandre Tsapin, 2000

机译：H蛋白在红螺螺旋藻光化学反应中心和细胞质膜组装中的作用
7. Defined covalent assembly of protein molecules onudgraphene using a genetically encoded photochemicaludreaction handle [O] . Zaki A, Hartley A, Reddington S, 100

机译：定义蛋白质分子的共价组装石墨烯使用遗传编码的光化学 ud反应手柄

Defined covalent assembly of protein molecules on graphene using a genetically encoded photochemical reaction handle

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