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UHPLC-ESI-Q-TOF-MS/MS analysis, antioxidant activity combined fingerprints for quality consistency evaluation of compound liquorice tablets

机译：UHPLC-ESI-Q-TOF-MS / MS分析，抗氧化活性组合指纹的复合液体片剂的优质一致性评价

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摘要

Traditional Chinese medicines (TCM)/herbal medicines (HM) are too complicated to comprehensively investigate their quality consistency effectively with a single detection technique. Hence, finding an effective, rapid, and comprehensive quality control (QC) method is of great importance for guaranteeing the safety and efficacy of TCM/HM in clinical applications. In our current research, a novel strategy of multi-wavelength fusion HPLC fingerprints and ultraviolet (UV) spectroscopic fingerprinting was proposed and successfully applied to monitor the quality consistency of compound liquorice tablets (CLT). The quality grades of 35 CLT samples from two manufacturers were successfully discriminated and evaluated by the averaged linear quantified fingerprint method (ALQFM) from a qualitative and quantitative perspective. The results showed that the UV spectroscopic fingerprints agreed well with the multi-wavelength fusion HPLC fingerprints. In addition, ultra-high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry (UHPLC-ESI-Q-TOF-MS) was applied to investigate the chemical constituents in CLT samples, providing an important chemical structural foundation for further QC and bioactivity studies. Additionally, a simple flow injection analysis (FIA) was developed to investigate the antioxidant capacity in CLT, which was based on the scavenging of 2,2-diphenyl-1-picrylhydrazyl radicals by antioxidants. Furthermore, the fingerprintefficacy relationship between high-performance liquid chromatography (HPLC) fingerprints and the antioxidant activities of CLT samples was established utilizing orthogonal projection to latent structures (OPLS). In conclusion, this study indicated that integrating UHPLC-ESI-Q-TOF-MS/MS, UV spectroscopic fingerprints, and multi-wavelength fusion HPLC fingerprints coupled with the antioxidant activities reported could give important clues for further pharmacological and clinical studies of CLT. Meanwhile, it provides a practical strategy for the rapid screening and identifying of TCM/HM quality consistency.
机译：中药（TCM）/草药（HM）太复杂，以通过单一检测技术综合研究其质量一致性。因此，寻找有效，快速，综合的质量控制（QC）方法非常重要，可保证TCM / HM在临床应用中的安全性和功效。在我们目前的研究中，提出了一种新颖的多波长融合HPLC指纹和紫外线（UV）光谱指纹识别并成功地应用于监测复合液体片剂（CLT）的质量稠度。由两个制造商的35个CLT样品的质量等级通过来自定性和定量的观点的平均线性定量指纹方法（ALQFM）成功地区分和评估。结果表明，UV光谱指纹与多波长融合HPLC指纹相同。此外，应用与电喷雾电离四极其飞行时间质谱（UHPLC-ESI-Q-TOF-MS）的超高效液相色谱法施用以研究CLT样品中的化学成分，提供重要的化学结构基础用于进一步的QC和生物活性研究。另外，开发了一种简单的流动注射分析（FIA）以研究CLT中的抗氧化能力，其基于通过抗氧化剂的2,2-二苯基-1-富铬基团的清除。此外，利用正交投影建立了高效液相色谱（HPLC）指纹和CLT样品的抗氧化活性的指纹差错关系。总之，本研究表明，与报道的抗氧化活动相结合的UHPLC-ESI-Q-TOF-MS / MS，UV光谱指纹和多波长融合HPLC指纹可以为CLT的进一步药理学和临床研究提供重要的线索。同时，它提供了快速筛选和识别TCM / HM质量一致性的实际策略。

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  • 来源
    《RSC Advances》 |2018年第49期|共13页
  • 作者

    Zhang Yujing; Wang Chao; Yang Fangliang; Yang Zhe; Wang Fangren; Sun Guoxiang;

  • 作者单位

    Shenyang Pharmaceut Univ Sch Pharm 103 Wenhua Rd Shenyang 110016 Liaoning Peoples R China;

    Shenyang Pharmaceut Univ Sch Pharmaceut Engn Shenyang Liaoning Peoples R China;

    Shenyang Pharmaceut Univ Sch Pharm 103 Wenhua Rd Shenyang 110016 Liaoning Peoples R China;

    Shenyang Pharmaceut Univ Sch Pharm 103 Wenhua Rd Shenyang 110016 Liaoning Peoples R China;

    Shenyang Pharmaceut Univ Sch Pharm 103 Wenhua Rd Shenyang 110016 Liaoning Peoples R China;

    Shenyang Pharmaceut Univ Sch Pharm 103 Wenhua Rd Shenyang 110016 Liaoning Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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