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首页> 外文期刊>RSC Advances >Development of a rapid and simple tetracycline detection system based on metal-enhanced fluorescence by europium-doped AgNP@SiO2 core-shell nanoparticles
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Development of a rapid and simple tetracycline detection system based on metal-enhanced fluorescence by europium-doped AgNP@SiO2 core-shell nanoparticles

机译：基于金属增强荧光的快速和简单的四环素检测系统的开发通过铕掺杂的AgNP〜SiO2核心壳纳米粒子

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摘要

We herein describe a rapid and selective sensing platform for tetracycline (Tc), which relies on the metal-enhanced fluorescence (MEF) effect of europium (Eu3+)-doped silver-silica core-shell nanoparticles (AgNP@SiO2). The developed assay utilizes AgNP@SiO2 as a key detection component, which is systematically optimized to have a silica shell thickness suitable for the effective MEF phenomenon. In principle, the AgNP@SiO2, which binds to Eu3+ through the electrostatic interaction, captures Tc by selective chelation with Eu3+, leading to significant fluorescence enhancement of the EuTc complex. Based on this novel strategy, we determined Tc as low as 83.1 nM with a total assay time of less than 10 min, which is comparable to or better than that of the previous fluorescence-based methods. Furthermore, the practical applicability of this strategy was successfully demonstrated by detecting Tc in tap water. This work highlights the unique features of AgNP@SiO2 for MEF-based biosensing applications.
机译：我们在本文中描述了一种用于四环素（TC）的快速和选择性的传感平台，依赖于金属增强型荧光（MEF）效应（EU3 +）掺杂的银 - 二氧化硅核 - 壳纳米粒子（AgNP @ SiO 2）。开发的测定利用AgNP @ SiO2作为关键检测组分，系统地优化为具有适合于有效MeF现象的二氧化硅壳厚度。原则上，通过静电相互作用与EU3 +结合的AgNP @ SiO 2通过选择性螯合与EU3 +捕获Tc，导致Eutc复合物的显着荧光增强。基于这种新的策略，我们确定了低至83.1nm的Tc，总测定时间不到10分钟，其比以前的基于荧光的方法更好或更好。此外，通过检测自来水中的TC成功证明了这种策略的实际适用性。这项工作突出了AGNP @ SiO2对于MEF的生物传感应用的独特功能。

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  • 来源
    《RSC Advances》 |2018年第43期|共6页
  • 作者

    Li Pei; Kumar Sathish; Park Ki Soo; Park Hyun Gyu;

  • 作者单位

    Korea Adv Inst Sci &

    Technol Dept Chem &

    Biomol Engn Program BK21 291 Daehak Ro Daejeon 34141 South Korea;

    Korea Adv Inst Sci &

    Technol Dept Chem &

    Biomol Engn Program BK21 291 Daehak Ro Daejeon 34141 South Korea;

    Konkuk Univ Coll Engn Dept Biol Engn Seoul 05029 South Korea;

    Korea Adv Inst Sci &

    Technol Dept Chem &

    Biomol Engn Program BK21 291 Daehak Ro Daejeon 34141 South Korea;

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  • 正文语种 eng
  • 中图分类 化学;
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