• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>RSC Advances >A statherin-derived peptide promotes hydroxyapatite crystallization and in situ remineralization of artificial enamel caries
【24h】

A statherin-derived peptide promotes hydroxyapatite crystallization and in situ remineralization of artificial enamel caries

机译：司法蛋白衍生的肽促进羟基磷灰石结晶，并原位再矿化对人工搪瓷龋齿

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In situ remineralization of hydroxyapatite on a human tooth enamel surface induced by anti-caries bioactive components is an alternative restorative strategy against dental caries. In this study, a novel biomimetic peptide DE-11, inspired by the salivary phosphoprotein statherin, was developed, and it showed beneficial potentials for the restoration of demineralized tooth enamel in vitro. The peptide DE-11 contained the initial six-peptide sequence of N-terminus of statherin extended by a mineralization hydrophilic tail composed of consecutive acidic amino acids capable of adsorbing calcium and phosphate ions. A strong adsorption capacity of DE-11 to hydroxyapatite was confirmed through Langmuir adsorption isotherm experiment and confocal laser scanning microscopy. Then, the nucleation and crystallization of hydroxyapatite due to DE-11 was characterized by scanning and transmission electron microscopy and selected-area electron diffraction. Moreover, the ability of DE-11 to promote the remineralization of initial enamel caries lesions was further evaluated. Initial lesions were created in bovine enamel blocks, which were then exposed to the peptide solution and finally immersed in artificial saliva. After 7 days, a higher percentage of surface microhardness recovery, a lower mineral loss, a shallower lesion depth, and a higher mineral content were found on the surface of the lesion body in the DE-11 group as compared to that in the negative group using surface microhardness testing, polarized light microscopy, and transverse microradiography; this indicated that DE-11 could induce in situ remineralization of hydroxyapatite on the demineralized enamel surface. Overall, these findings suggest that DE-11 is highly promising as a restorative biomaterial for enamel remineralization in the anti-caries applications.
机译：在抗龋生物活性组分诱导的人牙牙釉质表面上，羟基磷灰石的原位再矿化是针对龋齿的替代恢复策略。在这项研究中，一种新型的仿生肽DE-11，由唾液磷蛋白富酪蛋白的启发，被开发，并且它显示出有益的电位用于体外脱矿质牙齿珐琅质的恢复。肽DE-11含有由能够吸附钙和磷酸盐离子的连续酸性氨基酸组成的矿化亲水性延伸的初始六肽序列的初始六肽序列。通过Langmuir吸附等温实验和共聚焦激光扫描显微镜确认DE-11至羟基磷灰石的强烈吸附能力。然后，通过扫描和透射电子显微镜和选择区域电子衍射，表征羟基磷灰石的成核和结晶。此外，进一步评估了DE-11促进初始搪瓷龋病病变的再矿化的能力。在牛釉质嵌段中产生初始病变，然后暴露于肽溶液中并最终浸入人造唾液中。 7天后，与阴性组中的病变体中的病变体的表面上发现较高百分比的表面显微硬度恢复，较低的矿物质损失，较浅的病变深度和更高的矿物质含量使用表面微硬度测试，偏振光显微镜和横向微孔造影;这表明DE-11可以在脱尾牙釉质表面上促进羟基磷灰石的原位再矿化。总体而言，这些研究结果表明，DE-11在抗龋应用中的牙釉质再矿化是一种恢复性生物材料的高度令人欣然。

著录项

  • 来源
    《RSC Advances》 |2018年第3期|共9页
  • 作者

    Wang Kun; Wang Xiuqing; Li Haoran; Zheng Sainan; Ren Qian; Wang Yufei; Niu Yumei; Li Wei; Zhou Xuedong; Zhang Linglin;

  • 作者单位

    Sichuan Univ West China Hosp Stomatol Dept Cariol &

    Endodont Natl Clin Res Ctr Oral Dis State Key Lab Oral Dis Chengdu Sichuan Peoples R China;

    Sichuan Univ West China Hosp Stomatol Dept Cariol &

    Endodont Natl Clin Res Ctr Oral Dis State Key Lab Oral Dis Chengdu Sichuan Peoples R China;

    Sichuan Univ West China Hosp Stomatol Dept Cariol &

    Endodont Natl Clin Res Ctr Oral Dis State Key Lab Oral Dis Chengdu Sichuan Peoples R China;

    Sichuan Univ West China Hosp Stomatol Dept Cariol &

    Endodont Natl Clin Res Ctr Oral Dis State Key Lab Oral Dis Chengdu Sichuan Peoples R China;

    Sichuan Univ West China Hosp Stomatol Dept Cariol &

    Endodont Natl Clin Res Ctr Oral Dis State Key Lab Oral Dis Chengdu Sichuan Peoples R China;

    Sichuan Univ West China Hosp Stomatol Dept Cariol &

    Endodont Natl Clin Res Ctr Oral Dis State Key Lab Oral Dis Chengdu Sichuan Peoples R China;

    Sichuan Univ West China Hosp Stomatol Dept Cariol &

    Endodont Natl Clin Res Ctr Oral Dis State Key Lab Oral Dis Chengdu Sichuan Peoples R China;

    Sichuan Univ West China Hosp Stomatol Dept Cariol &

    Endodont Natl Clin Res Ctr Oral Dis State Key Lab Oral Dis Chengdu Sichuan Peoples R China;

    Sichuan Univ West China Hosp Stomatol Dept Cariol &

    Endodont Natl Clin Res Ctr Oral Dis State Key Lab Oral Dis Chengdu Sichuan Peoples R China;

    Sichuan Univ West China Hosp Stomatol Dept Cariol &

    Endodont Natl Clin Res Ctr Oral Dis State Key Lab Oral Dis Chengdu Sichuan Peoples R China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号