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Isolation of nitrate-reducing bacteria from an offshore reservoir and the associated biosurfactant production

机译：从海上储层和相关的生物活性剂生产中分离硝酸盐降低细菌

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摘要

Biosurfactant producing nitrate-reducing bacteria (NRB) in anaerobic reservoir environments are closely associated with souring (H2S) control in the offshore oil and gas industry. Five NRB strains were screened from offshore produced water samples and all were identified as Pseudomonas stutzeri. Their biosurfactant producing abilities when fed on either glucose or glycerol media were investigated. P. stutzeri CX3 reduced the medium surface tension to 33.5 and 29.6 mN m(-1), respectively, while growing on glucose or glycerol media. The CX3 strain was further inoculated to examine its growth performance, resulting in 32.4% and 94.5% of nitrate consumption over 228 hours of monitoring in two media, respectively. The composition analysis of the biosurfactant product generated by P. stutzeri CX3 was conducted through thin-layer chromatography, gas chromatography with a flame ionization detector (FID) and Fourier transform infrared spectroscopy (FT-IR). The biosurfactant product was identified as a mixture of a small part of lipopeptides and a large part of glycolipids while its critical micellar concentration (CMC) was as low as 35 mg L-1. The biosurfactant product demonstrated high stability over a wide range of temperature (4-121 degrees C), pH (2-10), and salinity (0-20% w/v) concentration. The results provided valuable technical and methodological support for effective offshore reservoir souring control and associated enhanced oil recovery activities.
机译：在厌氧储层环境中产生硝酸盐降低细菌（NRB）的生物活性剂与海上石油和天然气工业中的溶解（H2S）控制密切相关。从海上产生了五种NRB菌株，产生了水样，所有物质都被鉴定为假单胞菌斯图塞。研究了它们在葡萄糖或甘油培养基上喂食时的生物表面活性剂的能力。 P. Stutzeri CX3分别将培养基表面张力降低至33.5和29.6mN m（-1），同时在葡萄糖或甘油培养基上生长。进一步接种CX3菌株以检测其生长性能，导致32.4％和94.5％的硝酸盐消耗量分别在两种培养基中监测228小时。通过薄层色谱法，通过薄层色谱法，用火焰电离检测器（FID）和傅里叶变换红外光谱（FT-IR）进行薄层色谱法，通过薄层色谱法进行生物活性剂产品的组成分析。将生物活性剂产物鉴定为脂肽的一小部分的混合物和大部分糖脂，而其临界胶束浓度（CMC）低至35mg L-1。生物活性剂产品在宽范围的温度（4-121℃），pH（2-10）和盐度（0-20％w / v）浓度上显示出高稳定性。结果为有效的海上水库腐蚀控制和相关的溢油活动提供了有价值的技术和方法支持。

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  • 来源
    《RSC Advances》 |2018年第47期|共14页
  • 作者

    Fan Fuqiang; Zhang Baiyu; Morrill Penny L.; Husain Tahir;

  • 作者单位

    Mem Univ Newfoundland Northern Reg Persistent Organ Pollut Control NRPO Fac Engn &

    Appl Sci St John NF A1B 3X5 Canada;

    Mem Univ Newfoundland Northern Reg Persistent Organ Pollut Control NRPO Fac Engn &

    Appl Sci St John NF A1B 3X5 Canada;

    Mem Univ Newfoundland Earth Sci Fac Sci St John NF A1B 3X5 Canada;

    Mem Univ Newfoundland Northern Reg Persistent Organ Pollut Control NRPO Fac Engn &

    Appl Sci St John NF A1B 3X5 Canada;

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  • 正文语种 eng
  • 中图分类 化学;
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