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首页> 外文期刊>RSC Advances >Ultrasensitive fluorescent aptasensor for MUC1 detection based on deoxyribonuclease I-aided target recycling signal amplification
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Ultrasensitive fluorescent aptasensor for MUC1 detection based on deoxyribonuclease I-aided target recycling signal amplification

机译：超敏感荧光Aptasensor用于MUC1检测基于脱氧核酸核糖酶I辅助目标再循环信号放大

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摘要

A novel sensing strategy for sensitive detection of mucin 1 protein (MUC1) based on deoxyribonuclease I-aided target recycling signal amplification was proposed. In this paper, in the absence of MUC1, the MUC1 aptamer is absorbed on the surface of graphene oxide (GO) via pi-stacking interactions. This results in quenching of the fluorescent label and no fluorescence signal is observed. Upon adding MUC1, the probe sequences could be specifically recognized by MUC1, leading to an increase in the fluorescence intensity. The detection limit is as low as 10 pg mL(-1), and a linear range from 50 pg mL(-1) to 100 ng mL(-1). The assay is specific and sensitive, and successfully applied to the determination of MUC1 in spiked human serum, urine and saliva. Importantly, the proposed aptasensing strategy has great potential in detecting various protein and even cancer cells.
机译：提出了一种基于脱氧核糖核酸酶I-辅助靶再循环信号扩增的粘蛋白1蛋白（MUC1）敏感检测的新型感测策略。 本文在不存在MUC1的情况下，MUC1适体通过PI堆叠相互作用在石墨烯（GO）的表面上被吸收。 这导致荧光标记淬火并且未观察到荧光信号。 在添加MUC1后，可以通过MUC1具体识别探针序列，导致荧光强度的增加。 检出限量低至10pg ml（-1），线性范围为50pg ml（-1）至100ng ml（-1）。 该测定是特异性和敏感的，并且成功地应用于尖刺人血清，尿液和唾液中的MUC1的测定。 重要的是，所提出的Aptasening策略在检测各种蛋白质和甚至癌细胞中具有很大的潜力。

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  • 来源
    《RSC Advances》 |2018年第56期|共7页
  • 作者

    Zhang Jun; Ran Fengying; Zhou Wenbo; Shang Bing; Yu Fei; Wu Lun; Hu Wanbao; He Xueqin; Chen Qinhua;

  • 作者单位

    Hubei Univ Med Affiliated Dongfeng Hosp Shiyan 442008 Hubei Peoples R China;

    Hubei Univ Med Affiliated Dongfeng Hosp Shiyan 442008 Hubei Peoples R China;

    Hubei Univ Med Affiliated Dongfeng Hosp Shiyan 442008 Hubei Peoples R China;

    Hubei Univ Med Affiliated Dongfeng Hosp Shiyan 442008 Hubei Peoples R China;

    Hubei Univ Med Affiliated Dongfeng Hosp Shiyan 442008 Hubei Peoples R China;

    Hubei Univ Med Affiliated Dongfeng Hosp Shiyan 442008 Hubei Peoples R China;

    Sinopharm Dongfeng Huaguo Hosp Shiyan 442008 Hubei Peoples R China;

    Hubei Univ Med Affiliated Dongfeng Hosp Shiyan 442008 Hubei Peoples R China;

    Hubei Univ Med Affiliated Dongfeng Hosp Shiyan 442008 Hubei Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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