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Analysis of binding properties of pathogens and toxins using multivalent glycan microarrays

机译：使用多价甘草微阵列分析病原体和毒素的结合特性

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摘要

Pathogens infect hosts often through initial binding of their cell surface lectins to glycans expressed on the exterior of host cells. Thus, methods to evaluate the glycan-binding properties of pathogens are of great importance. Because of the multivalent nature of interactions of pathogens with glycans, the ability to assess the glycan density-dependent binding of pathogens is particularly important. In this study, we developed a facile technique to construct multivalent carbohydrate microarrays through immobilization of unmodified glycans on multivalent hydrazide-derivatized glass surfaces. This immobilization strategy does not require the use of multivalent glycoconjugates, which are typically prepared by using multistep sequences. The results of analysis of microarray images, obtained after incubation of multivalent glycan microarrays with cholera toxin B and pathogens such as uropathogenic E. coli and H. pylori, show that the binding affinities of toxins and pathogens for glycans are highly glycan density-dependent. Specifically, toxins and pathogens bind to glycans more strongly as the valency of the glycans on the microarrays is increased from 1 to 4. It is anticipated that the newly developed immobilization method will be applicable to the preparation of multivalent carbohydrate microarrays that are employed to evaluate multivalent glycan binding properties of a variety of pathogens and toxins.
机译：病原体通常通过其细胞表面凝集素对宿主细胞外部表达的聚糖的初始结合来感染宿主。因此，评价病原体的甘草结合特性的方法非常重要。由于病原体与聚糖的相互作用的多价性质，评估甘油密度依赖性病原体的能力尤为重要。在这项研究中，我们开发了一种通过固定在多价酰肼 - 衍生玻璃表面上的未改性的聚糖来构建多价碳水化合物微阵列的容纳技术。这种固定策略不需要使用多价糖缀合物，其通常通过使用多步序列来制备。在用霍乱毒素B和病原体孵育多价甘油微阵列的微阵列图像分析结果，例如尿鼠胆汁大肠杆菌和H.幽门螺杆菌，表明聚糖的毒素和病原体的结合亲和力是高度甘油密度依赖性的。具体而言，随着微阵列上的聚糖的化合物从1-4增加，毒素和病原体更强烈地结合聚糖和病原体。预计新开发的固定方法将适用于制备用于评估的多价碳水化合物微阵列。多元聚糖的各种病原体和毒素的结合特性。

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  • 来源
    《RSC Advances》 |2018年第27期|共8页
  • 作者

    Kim Hyoung Sub; Hyun Ji Young; Park Seong-Hyun; Shin Injae;

  • 作者单位

    Yonsei Univ Dept Chem Ctr Biofunct Mol Seoul 03722 South Korea;

    Yonsei Univ Dept Chem Ctr Biofunct Mol Seoul 03722 South Korea;

    Yonsei Univ Dept Chem Ctr Biofunct Mol Seoul 03722 South Korea;

    Yonsei Univ Dept Chem Ctr Biofunct Mol Seoul 03722 South Korea;

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  • 正文语种 eng
  • 中图分类 化学;
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