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首页> 外文期刊>RSC Advances >Development of a Pichia pastoris whole-cell biocatalyst with overexpression of mutant lipase I PCLG47I from Penicillium cyclopium for biodiesel production
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Development of a Pichia pastoris whole-cell biocatalyst with overexpression of mutant lipase I PCLG47I from Penicillium cyclopium for biodiesel production

机译：从Penicillium Cyner opy的突变体脂肪酶I Pclg47i进行生物柴油生产的Pichia Pastoris全细胞生物催化剂的开发

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摘要

Penicillium cyclopium lipase I (PCL) is a thermolabile triacylglycerol lipase with very low activity against monoacylglycerols, and there have been no reports on the transesterification of oil to produce biodiesel. A mutant PCLG47I with an improved thermostability was previously obtained through replacing Gly47 with Ile in PCL. In this study, a novel Pichia pastoris whole-cell biocatalyst (WCB) with overexpression of PCLG47I was constructed and characterized for biodiesel production from soybean oil. The optimum conditions for biodiesel preparation were 1 g soybean oil, 1 : 2 initial oil/methanol molar ratio with 3 times methanol addition of 1 : 0.75 oil/methanol molar ratio at 4 h intervals, 7% water content, 400 U lipase, temperature of 25 degrees C, and reaction time of 20 h. Under the optimum conditions, the FAME yield reached 60.7% and remained 47.3% after 4 batch cycles, and no glycerol was generated as a byproduct. These findings indicated that this WCB is a promising biocatalyst for biodiesel production in a relatively cost-effective manner. Additionally, the resulting enzymatic process may provide a potential method for biodiesel production at an industrial scale.
机译：Penicillium脂肪酶I（PCL）是一种具有非常低的单酰基甘油活性的热型三酰基甘油脂肪酶，并没有关于油的酯交换产生生物柴油的报道。先前通过用PCL中用ILE替换GLY47来获得具有改善的热稳定性的突变PCLG47I。在本研究中，构建了具有PCLG47i过表达的小型毕赤酵母的全细胞生物催化剂（WCB），并表征来自大豆油的生物柴油生产。生物柴油制剂的最佳条件为1g豆油，1：2初始油/甲醇摩尔比，3次加入1：0.75油/甲醇摩尔比以4小时间隔，7％含水量，400 u脂肪酶，温度25℃，反应时间为20小时。在最佳条件下，由于4个分批循环后，增物产率达到60.7％，持续47.3％，并且不会产生甘油作为副产物。这些发现表明，这种WCB是一种具有相对成本效益的生物柴油生产的有前途的生物催化剂。另外，所得酶促方法可以在工业规模提供生物柴油生产的潜在方法。

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  • 来源
    《RSC Advances》 |2018年第46期|共8页
  • 作者

    Liu Yihan; Huang Lin; Zheng Dong; Fu Yu; Shan Mengying; Xu Zehua; Ma Jieying; Lu Fuping;

  • 作者单位

    Minist Educ Key Lab Ind Fermentat Microbiol Tianjin 300457 Peoples R China;

    Minist Educ Key Lab Ind Fermentat Microbiol Tianjin 300457 Peoples R China;

    Minist Educ Key Lab Ind Fermentat Microbiol Tianjin 300457 Peoples R China;

    Minist Educ Key Lab Ind Fermentat Microbiol Tianjin 300457 Peoples R China;

    Tianjin Key Lab Ind Microbiol Tianjin 300457 Peoples R China;

    Tianjin Key Lab Ind Microbiol Tianjin 300457 Peoples R China;

    Tianjin Key Lab Ind Microbiol Tianjin 300457 Peoples R China;

    Minist Educ Key Lab Ind Fermentat Microbiol Tianjin 300457 Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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