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首页> 外文期刊>RSC Advances >An immunochromatographic dipstick as an alternate for monitoring of heroin metabolites in urine samples
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An immunochromatographic dipstick as an alternate for monitoring of heroin metabolites in urine samples

机译：免疫粒子分离性Dipstick作为监测尿液样本中海洛因代谢物的交替

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摘要

Heroin use and addiction pose serious risks and side effects due to overdose. Quantification of heroin in biological samples is challenging due to rapid deacetylation of heroin to its active metabolites. In this study, we report the quantification of metabolic degradation of heroin by-products in biological urine samples. The presence of the drug was monitored after oral administration of heroin at different time intervals. Various biophysical techniques, such as high performance liquid chromatography (HPLC) and mass spectrometry (MS) were used to evaluate the presence of the drug. A competitive fluorescence based immunoassay was developed with a limit of detection (LOD) up to 0.01 ng mL(-1) and the IC50 value was 0.1 ng mL(-1), while the dipstick assay shows a LOD up to 5 ng mL(-1). Rapid detection of narcotic drugs was carried out for biological urine samples collected at various time points. Validation of the developed dipstick was carried out for the standard as well as the spiked urine samples by fluorescence based immunoassay (FIA), using anti-morphine antibodies. A strong correlation (R = 0.94) was obtained between the developed dipstick and FIA assay for biological urine samples collected at various time points. The developed immunochromatographic dipstick is highly sensitive, field applicable and cost effective, and can serve as a first choice for the monitoring of narcotic drugs in blood, urine and saliva in drug addicts and athletes.
机译：海洛因使用和成瘾构成了由于过量的严重风险和副作用。由于海洛因快速脱乙酰地区在其活性代谢物中，海洛因在生物样品中的定量是挑战性的。在这项研究中，我们报告了在生物尿液样本中的海洛因副产物的代谢降解量化。在口服以不同的时间间隔口服施用海洛因后监测药物的存在。使用各种生物物理技术，例如高效液相色谱（HPLC）和质谱（MS）来评估药物的存在。竞争的荧光基于荧光的免疫测定具有高达0.01 ng ml（-1）的检测限（LOD），并且IC 50值为0.1ng mL（-1），而Dipstick测定显示高达5 ng ml的含量（ -1）。在各种时间点收集的生物尿液样本进行了快速检测麻醉药。使用抗吗啡抗体，对标准的验证以及荧光的免疫测定（FIA）进行标准，以及尖刺的尿液样本进行。在不同时间点收集的生物尿液样本之间产生强烈的相关性（R = 0.94）。发达的免疫粒状降级高度敏感，现场适用和成本效益，并且可以作为监测吸毒者和运动员中血液，尿液和唾液中麻醉药物的首选。

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  • 来源
    《RSC Advances》 |2018年第41期|共8页
  • 作者

    Mishra Priya; Banga Ivneet; Tyagi Roshika; Munjal Tanya; Goel Aditya; Capalash Neena; Sharma Prince; Suri C. R.; Gandhi Sonu;

  • 作者单位

    Amity Univ Amity Inst Biotechnol AIB Sect 125 Noida 201313 India;

    Amity Univ Amity Inst Biotechnol AIB Sect 125 Noida 201313 India;

    Amity Univ Amity Inst Biotechnol AIB Sect 125 Noida 201313 India;

    Amity Univ Amity Inst Biotechnol AIB Sect 125 Noida 201313 India;

    Amity Univ Amity Inst Biotechnol AIB Sect 125 Noida 201313 India;

    Panjab Univ Dept Biotechnol &

    Microbiol Sect 14 Chandigarh 160014 India;

    Panjab Univ Dept Biotechnol &

    Microbiol Sect 14 Chandigarh 160014 India;

    CSIR Inst Microbial Technol Sect 39A Chandigarh 160036 India;

    Amity Univ Amity Inst Biotechnol AIB Sect 125 Noida 201313 India;

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  • 正文语种 eng
  • 中图分类 化学;
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