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首页> 外文期刊>RSC Advances >The enhanced angiogenesis effect of VEGF-silk fibroin nanospheres-BAMG scaffold composited with adipose derived stem cells in a rabbit model
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The enhanced angiogenesis effect of VEGF-silk fibroin nanospheres-BAMG scaffold composited with adipose derived stem cells in a rabbit model

机译：VEGF-丝纤维素纳米型纳米蛋白纳米球的增强血管生成效应与兔模型中脂肪衍生的干细胞组合的脂肪衍生的干细胞

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摘要

We report a study to determine whether a vascular endothelial growth factor (VEGF)-silk fibroin (SF) nanospheres-bladder acellular matrix graft (BAMG) scaffold composited with adipose derived stem cells (ADSCs) could enhance angiogenesis in bladder regeneration in rabbits. Rabbit ADSCs were isolated and identified by flow cytometry. The morphology and release behaviour of VEGF-SF nanospheres were detected. After the composite scaffolds were successfully used in bladder reconstruction, the bladder capacity, H&E staining and immunohistochemical staining were studied at different time points. ADSCs exerts high expression rates of CD29, CD90, and CD44, accompanied with low expression rates of CD34 and CD45. SF nanospheres with diameters of 200-1000 nm were prepared to load VEGF, and they contributed to maintain the release of VEGF. The reconstructed bladder with VEGF-SF nanospheres-BAMG plus ADSCs had more regular smooth muscle tissue and blood vessels. Moreover, instead of differentiating into epithelial or vascular endothelial cells, ADSCs may be more likely to provide additional cytokines to enhance angiogenesis in the bladder regeneration process. The tissue engineered bladder constructed by BAMG modified by VEGF-SF nanospheres possessed high bio-compatibility and an enhanced angiogenesis effect, and could be used as an ideal biological material to repair bladder defects after being composited with ADSCs.
机译：我们报告一个研究，以确定一个血管内皮生长因子（VEGF）-silk素蛋白（SF）纳米球囊脱细胞基质接枝（BAMG）与脂肪来源的干细胞（ADSC中）复合支架是否能在家兔增强血管生成的膀胱再生。兔脂肪干细胞进行分离，并通过流式细胞术鉴定。检测到VEGF-SF纳米球的形态和释放行为。的复合支架在膀胱重建成功使用后，将膀胱容量，H＆E染色和免疫组织化学染色在不同时间点分析。脂肪干细胞发挥CD29，CD90和CD44的高表达率，并伴有CD34和CD45的低表达率。制备直径为200-1000纳米的纳米球SF加载VEGF，并且它们有助于维持VEGF的释放。与VEGF-SF纳米球，BAMG加脂肪干细胞重建膀胱有更多的定期平滑肌组织和血管。此外，代替分化为上皮细胞或血管内皮细胞，脂肪干细胞更可能提供额外的细胞因子，以增强血管生成在膀胱再生过程。通过BAMG构成的组织工程膀胱改性由VEGF-SF纳米球具有高的生物相容性和增强的血管生成作用，并与脂肪干细胞被合成后，可作为一种理想的生物材料，以修复膀胱缺陷。

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  • 来源
    《RSC Advances》 |2018年第27期|共8页
  • 作者

    Zhang Dongliang; Cao Nailong; Zhou Shukui; Chen Zhong; Zhang Xinru; Zhu Weidong;

  • 作者单位

    Shanghai Jiao Tong Univ Sch Med Shanghai Peoples Hosp 6 Dept Urol 600 Yishan Rd Shanghai 200233 Peoples R China;

    Shanghai Jiao Tong Univ Sch Med Shanghai Peoples Hosp 6 Dept Urol 600 Yishan Rd Shanghai 200233 Peoples R China;

    Shanghai Jiao Tong Univ Sch Med Shanghai Peoples Hosp 6 Dept Urol 600 Yishan Rd Shanghai 200233 Peoples R China;

    Shanghai Jiao Tong Univ Sch Med Shanghai Peoples Hosp 6 Dept Urol 600 Yishan Rd Shanghai 200233 Peoples R China;

    Shanghai Jiao Tong Univ Sch Med Shanghai Peoples Hosp 6 Dept Urol 600 Yishan Rd Shanghai 200233 Peoples R China;

    Shanghai Jiao Tong Univ Sch Med Shanghai Peoples Hosp 6 Dept Urol 600 Yishan Rd Shanghai 200233 Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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