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首页> 外文期刊>RSC Advances >Photoluminescent F-doped carbon dots prepared by ring-opening reaction for gene delivery and cell imaging
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Photoluminescent F-doped carbon dots prepared by ring-opening reaction for gene delivery and cell imaging

机译：通过开环反应制备的光致发光F掺杂的碳点，用于基因递送和细胞成像

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摘要

Carbon dots (CDs) are photoluminescent nanoparticles with distinctive properties, having great potential in nano-biomaterial systems such as gene/drug delivery vectors and cell imaging agents. Fluorine-doped CD C-6F was prepared by a one-step ring-opening polymerization-dehydrative carbonization (RPDC) approach based on low molecular weight polyethyleneimine (PEI, 600 Da) and fluorinated diglycidyl ethers, while the non-fluorinated counterpart C-6H and the CD prepared from PEI 600 Da solely (C-600) were also prepared for comparison. TEM, FT-IR and XPS were performed to determine the compositions and surface states of the CDs. In vitro cell experiment results reveal that the CDs prepared from RPDC approach have much higher transfection efficiency and cellular uptake than PEI 600 contrasts in various cell lines. Compared to non-fluorinated C-6H, C-6F exhibited distinctly higher transfection efficiency, and up to 30 and 260 times higher efficiency than PEI 25 kDa could be achieved in the absence and presence of serum, respectively, indicating the advantage of F-doping. Besides, these CDs exhibit good cell imaging capability under single wavelength excitation, making the materials suitable for cellular tracking and transfection mechanism studies. These results demonstrate that fluorine-doping is an efficient approach to obtained CD gene vectors with high efficiency and serum tolerance.
机译：碳点（CDS）是具有独特性性质的光致发光纳米颗粒，具有较大的纳米生物材料系统潜力，例如基因/药物递送载体和细胞成像剂。通过基于低分子量聚乙烯亚胺（PEI，600Da）和氟化二缩水甘油醚的一步开环聚合 - 脱水碳化（RPDC）方法制备氟掺杂的Cd C-6F，而非氟化对手C- 6H和由PEI 600Da制备的CD仅（C-600）制备用于比较。进行TEM，FT-IR和XPS以确定CD的组合物和表面状态。体外细胞实验结果表明，由RPDC方法制备的CD具有更高的转染效率和比PEI 600在各种细胞系中的对比。与非氟化C-6H相比，C-6F表现出明显较高的转染效率，并且在血清的情况下，可以在没有血清的情况下实现比PEI 25kDA的效率高达30和260倍，表明F-的优点兴奋剂。此外，这些CD在单波长激发下表现出良好的细胞成像能力，使得适用于细胞跟踪和转染机制研究的材料。这些结果表明，氟掺杂是获得高效率和血清耐受性的CD基因载体的有效方法。

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  • 来源
    《RSC Advances》 |2018年第11期|共10页
  • 作者

    Luo Tian-Ying; He Xi; Zhang Ji; Chen Ping; Liu Yan-Hong; Wang Hai-Jiao; Yu Xiao-Qi;

  • 作者单位

    Sichuan Univ Coll Chem Minist Educ Key Lab Green Chem &

    Technol Chengdu 610064 Sichuan Peoples R China;

    Sichuan Univ Coll Chem Minist Educ Key Lab Green Chem &

    Technol Chengdu 610064 Sichuan Peoples R China;

    Sichuan Univ Coll Chem Minist Educ Key Lab Green Chem &

    Technol Chengdu 610064 Sichuan Peoples R China;

    Sichuan Univ Coll Chem Minist Educ Key Lab Green Chem &

    Technol Chengdu 610064 Sichuan Peoples R China;

    Sichuan Univ Coll Chem Minist Educ Key Lab Green Chem &

    Technol Chengdu 610064 Sichuan Peoples R China;

    Sichuan Univ Coll Chem Minist Educ Key Lab Green Chem &

    Technol Chengdu 610064 Sichuan Peoples R China;

    Sichuan Univ Coll Chem Minist Educ Key Lab Green Chem &

    Technol Chengdu 610064 Sichuan Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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