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首页> 外文期刊>RSC Advances >Antifungal mechanism of cinnamaldehyde and citral combination against Penicillium expansum based on FT-IR fingerprint, plasma membrane, oxidative stress and volatile profile
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Antifungal mechanism of cinnamaldehyde and citral combination against Penicillium expansum based on FT-IR fingerprint, plasma membrane, oxidative stress and volatile profile

机译：基于FT-IR指纹，血浆膜，氧化应力和挥发性曲线的肉桂醛和Citral组合对青霉突变的抗真菌机制

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摘要

Cinnamaldehyde (Cin) and citral (Cit) have been studied as antimicrobial agents and natural preservatives, but their action modes are controversial, and the knowledge of their antifungal mechanism against P. expansum is still incomplete. The present study was conducted to evaluate the antifungal mechanism of the combination of Cin and Cit (Cin/Cit) against P. expansum by observing the cellular ultrastructure, fourier transform infrared spectroscopy (FT-IR) fingerprints, plasma membrane, oxidative stress and volatile profile. Cin/Cit caused membrane invaginations, organelles and cytoplasm destruction, as shown by transmission electron microscopy (TEM) observations. The FT-IR spectra and followed principle component analysis (PCA) presented the significant differences in chemical compounds, particularly phospholipid, protein and fatty acids of cells exposed to Cin/Cit. Compared to controls, Cin/Cit induced a decrease of ergosterol by 39.40%, an increase of unsaturated fatty acid, and protein release level (3.5 times). Besides, membrane damage was further verified through the reduction of the membrane integrity by using a flow cytometer. Moreover, the increase of malondialdehyde (MDA) (40.09%) and reactive oxygen species (ROS) accumulation indicated an induction of oxidative stress in cells by Cin/Cit. To resist the unfavorable stress caused by Cin/Cit, P. expansum metabolized Cin or Cit to the predominant detoxification compounds, cinnamic alcohol, nerol, and geraniol. The alterations in volatile profile demonstrated the influences on specific metabolisms in P. expansum caused by Cin/Cit.
机译：肉桂醛（CIN）和CITRAL（CIT）已被研究为抗微生物剂和天然防腐剂，但它们的动作模式是有争议的，以及对P.扩展的抗真菌机制的了解仍然不完整。通过观察蜂窝超微结构，傅里叶变换红外光谱（FT-IR）指纹，质膜，氧化应力和挥发性，进行本研究以评估CIN和CIT（CIN / CIT）的组合对P.扩展的抗真菌机制。傅立叶变换红外光谱（FT-IR）指纹，血浆膜，氧化应力和挥发性轮廓。 CIN / CIT引起膜侵略性，细胞器和细胞质破坏，如透射电子显微镜（TEM）观察所示。 FT-IR光谱和遵循的原理成分分析（PCA）呈现了化学化合物的显着差异，特别是磷脂，蛋白质和暴露于CIN / CIT的细胞的脂肪酸。与对照组相比，CIN / CIT诱导Ergosterol的减少39.40％，增加不饱和脂肪酸和蛋白质释放水平（3.5倍）。此外，通过使用流式细胞仪通过减少膜完整性进一步验证膜损伤。此外，丙二醛（MDA）（40.09％）和反应性氧（ROS）积累的增加表明CIN / CIT诱导细胞中氧化应激。为了抵抗Cin / CIT，P.eapanyum代谢CIN或CIT引起的不利应激，以占主导地解毒化合物，肉桂醇，甘醇和小苯二醇。挥发性型材的改变证明了CIN / CIT引起的P.扩张中的特异性代谢的影响。

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  • 来源
    《RSC Advances》 |2018年第11期|共10页
  • 作者

    Wang Yuan; Feng Kewei; Yang Haihua; Yuan Yahong; Yue Tianli;

  • 作者单位

    Northwest Univ Coll Food Sci &

    Engn Xian 710069 Shaanxi Peoples R China;

    Northwest A&

    F Univ Coll Agron State Key Lab Crop Stress Biol Arid Areas Yangling 712100 Peoples R China;

    Northwest A&

    F Univ Coll Food Sci &

    Engn Yangling 712100 Shaanxi Peoples R China;

    Northwest A&

    F Univ Coll Food Sci &

    Engn Yangling 712100 Shaanxi Peoples R China;

    Northwest Univ Coll Food Sci &

    Engn Xian 710069 Shaanxi Peoples R China;

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