A simple fluorescent assay for cyromazine detection in raw milk by using CYR-stabilized G-quadruplex formation

Xing Haibo; Gu Wenchao; Xu Dang; Tian Fuxiang; Yao Linyun; Wang Zhenwei; Hu Xiaojun

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A simple fluorescent assay for cyromazine detection in raw milk by using CYR-stabilized G-quadruplex formation

机译：通过使用Cyr稳定的G-Quadreplem倒栽，对原料乳中的氯胺检测的简单荧光测定

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A rapid biosensor for the detection of cyromazine in milk is reported based on a fluorescence quenching result. When an FAM labelled G-rich ssDNA Tcy2 is treated with cyromazine, it can form a G-quadruplex-CYR complex and cause a change in fluorescence. As a result, the presence of cyromazine can be determined by fluorescence quenching. This sensor is selective for the detection of cyromazine in raw milk and has a limit of detection of 0.68 ppb and a detection range from 0 to 200 ppb.

机译：报告基于荧光猝灭结果报告用于检测牛奶中氯胺的快速生物传感器。当用Cyromazine处理FAM标记的GR富含的GRIFSDNA TCY2时，它可以形成G-QuadRuple-Cyr络合物并导致荧光的变化。结果，通过荧光猝灭可以确定Cyromazine的存在。该传感器是对原料乳中氯胺的检测选择性的选择性，并且检测到0.68ppb的检测限，检测范围为0至200ppb。

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来源
《RSC Advances》 |2018年第5期|共8页
作者
Xing Haibo; Gu Wenchao; Xu Dang; Tian Fuxiang; Yao Linyun; Wang Zhenwei; Hu Xiaojun;
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作者单位

Shanghai Inst Technol Sch Chem &

Environm Engn Shanghai 201418 Peoples R China;

Putuo Dist Ctr Dis Control &

Prevent Shanghai Peoples R China;

Shanghai Inst Technol Sch Chem &

Environm Engn Shanghai 201418 Peoples R China;

Shanghai Inst Technol Sch Chem &

Environm Engn Shanghai 201418 Peoples R China;

Shanghai Inst Technol Sch Perfume &

Aroma Technol Shanghai 201418 Peoples R China;

Shanghai Inst Technol Sch Chem &

Environm Engn Shanghai 201418 Peoples R China;

Shanghai Inst Technol Sch Chem &

Environm Engn Shanghai 201418 Peoples R China;

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正文语种 eng
中图分类化学;
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1. A simple fluorescent assay for cyromazine detection in raw milk by using CYR-stabilized G-quadruplex formation [J] . Xing Haibo, Gu Wenchao, Xu Dang, RSC Advances . 2018,第5期

机译：通过使用Cyr稳定的G-Quadreplem倒栽，对原料乳中的氯胺检测的简单荧光测定
2. A Rapid and Sensitive Aptasensor for Cyromazine Detection in Raw Milk Based on a Nanogold Probe and G-Quadruplex Formation [J] . Dang Xu, Gu Wenchao, Zheng Xiyin, Australian Journal of Chemistry: A Journal for the Publication of Original Research in All Branches of Chemistry . 2019,第7期

机译：基于纳米型探针和G-Quadreplex形成的原料牛奶中碱性碱性检测的快速敏感的Aptasensor
3. A label-free fluorescent turn-on enzymatic amplification assay for DNA detection using ligand-responsive G-quadruplex formationf [J] . Chuanqi Zhao, Li Wu, Jinsong Ren, Chemical Communications . 2011,第19期

机译：使用配体响应性G-四链体形成物进行DNA检测的无标记荧光开启酶促扩增测定法f
4. STANDARDIZATION OF BIOLUMINESCENT RAW MILK QUALITY ASSAY USING LUMTEK TEST-SYSTEMS [C] . VG FRUNDZHYAN, NN UGAROVA, AA MAKARUSHIN, International Symposium on Bioluminescence and Chemiluminescence . 2007

机译：使用LUMTEK测试系统的生物发光原料乳质质量测定的标准化
5. Study of a Bacillus circulans chitin -binding domain by a green fluorescent protein binding assay and detection of lysozymes by improved zymograms. [D] . Hardt, Markus. 2002

机译：通过绿色荧光蛋白结合测定法研究环生芽孢杆菌的几丁质结合结构域，并通过改进的酶谱图检测溶菌酶。
6. Label-free detection of kanamycin based on a G-quadruplex DNA aptamer-based fluorescent intercalator displacement assay [O] . Yun-Peng Xing, Chun Liu, Xiao-Hong Zhou, -1

机译：基于G-四链体DNA适体的荧光嵌入剂置换试验无标记地检测卡那霉素
7. A simple fluorescent assay for cyromazine detection in raw milk by using CYR-stabilized G-quadruplex formation [O] . Haibo Xing, Wenchao Gu, Dang Xu, 2018

机译：通过使用Cyr稳定的G-Quadreplem倒栽，对原料乳中的氯胺检测的简单荧光测定

A simple fluorescent assay for cyromazine detection in raw milk by using CYR-stabilized G-quadruplex formation

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