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首页> 外文期刊>RSC Advances >Efficient promiscuous Knoevenagel condensation catalyzed by papain confined in Cu-3(PO4)(2) nanoflowers
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Efficient promiscuous Knoevenagel condensation catalyzed by papain confined in Cu-3(PO4)(2) nanoflowers

机译：用木瓜蛋白酶催化在Cu-3（PO4）（2）纳米割草机中催化的高效XoevenageL凝聚

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摘要

To develop an efficient and green immobilized biocatalyst for promiscuous catalysis which has a broad scope of applications, hybrid nanoflower (hNF) confined papain as a biocatalyst has been proposed and characterized in this study. hNFs were firstly prepared through mixing CuSO4 aqueous solution with papain in phosphate saline (PBS) at room temperature. The resulting hNFs were characterized by SEM and verified through a hydrolysis reaction with N-benzoyl-DL-arginine amide as substrate. Under optimal conditions, this nano-biocatalyst demonstrated a 15-fold hydrolytic activity compared with papain of free form, along with better thermal stability. A series of reaction factors (reaction temperature, time, and solvent) have been investigated for Knoevenagel condensation reactions with hNFs as catalyst. At optimal conditions, product yield of the hNFs catalyzed reaction was 1.3 fold higher than that of the free enzyme with benzaldehyde and acetylacetone as substrates. A few aldehydes and methylene compounds have also been used to test the generality and scope of this new enzymatic promiscuity. To sum up, the obtained hNFs demonstrate better catalytic properties than free papain and the inorganic metal-salt crystal can function as both support and promotor in biocatalysis.
机译：为了开发一种具有广泛应用范围的杂交催化剂的高效和绿色固定的生物催化剂，杂交纳米橄榄润（HNF）被限制为生物催化剂，并在本研究中表征。首先通过在室温下将CuSO4水溶液与磷酸盐（PBS）混合的CuSO4水溶液混合来制备HNF。通过SEM进行所得的HNF，并通过与N-苯甲酰-D1-精氨酸酰胺作为基质的水解反应来验证。在最佳条件下，该纳米生物催化剂与自由形式的木瓜蛋白质相比，该纳米生物催化剂展示了15倍的水解活性，以及​​更好的热稳定性。已经研究了一系列反应因子（反应温度，时间和溶剂），用于与HNFS作为催化剂的KnoevenageL缩合反应。在最佳条件下，HNFS催化反应的产物产率比与苯甲醛和乙酰丙酮作为基材的游离酶的产物产量高1.3倍。还用于测试这种新的酶促滥交的一般性和范围的少数醛和亚二甲基化合物。总而言之，所得的HNFS表明比游离木瓜蛋白酶更好的催化性质，无机金属 - 盐晶可以用作生物分析中的支持和促进剂。

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  • 来源
    《RSC Advances》 |2018年第5期|共8页
  • 作者

    Yu Jianyun; Chen Xinxin; Jiang Min; Wang Anming; Yang Linlin; Pei Xiaolin; Zhang Pengfei; Wu Stephen Gang;

  • 作者单位

    Hangzhou Normal Univ Coll Mat Chem &

    Chem Engn Hangzhou 310014 Zhejiang Peoples R China;

    Hangzhou Normal Univ Coll Mat Chem &

    Chem Engn Hangzhou 310014 Zhejiang Peoples R China;

    Hangzhou Normal Univ Coll Mat Chem &

    Chem Engn Hangzhou 310014 Zhejiang Peoples R China;

    Hangzhou Normal Univ Coll Mat Chem &

    Chem Engn Hangzhou 310014 Zhejiang Peoples R China;

    Hangzhou Normal Univ Coll Mat Chem &

    Chem Engn Hangzhou 310014 Zhejiang Peoples R China;

    Hangzhou Normal Univ Coll Mat Chem &

    Chem Engn Hangzhou 310014 Zhejiang Peoples R China;

    Hangzhou Normal Univ Coll Mat Chem &

    Chem Engn Hangzhou 310014 Zhejiang Peoples R China;

    Washington Univ Dept Energy Environm &

    Chem Engn St Louis MO 63130 USA;

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