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Application of a cation-exchange reaction of CuS nanoparticles and fluorescent copper nanoparticles in a DNA biosensor

机译：CUS纳米粒子和荧光铜纳米粒子在DNA生物传感器中的应用

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摘要

A novel detection method based on the cation-exchange reaction of CuS nanoparticles (CuS NPs) combined with poly T-templated fluorescent Cu nanoparticles (Cu NPs) was developed. First, CuS NPs-magnetic bead conjugates were prepared through the hybridization of DNA. Competition with target DNA resulted in the release of CuS NPs, and exonuclease III catalysis could lead to recycling of the target DNA. Then, the CuS NPs released into the supernatant were subjected to a cation-exchange reaction after the addition of AgNO3. The obtained Cu2+ could form fluorescent Cu NPs using poly T DNA as a template. The fluorescence intensity of the Cu NPs could be used to determine the concentration of the target DNA. To further increase the detection sensitivity, two types of DNA decorated magnetic beads were used. After Exo III digestion for two cycle processes, more CuS NPs entered the supernatant. Hence, a stronger fluorescence intensity was found after the cation-exchange reaction and the formation of fluorescent Cu NPs. The developed method is convenient and low cost with good sensitivity and selectivity.
机译：开发了一种基于CUS纳米颗粒（CUS NPS）的新型检测方法与聚T型荧光荧光Cu纳米颗粒（Cu NPS）组合。首先，通过DNA的杂交制备CUS NPS-磁珠缀合物。与靶DNA的竞争导致CUS NPS的释放，外切核酸酶III催化可能导致靶DNA的再循环。然后，在加入AgNO 3后，对释放到上清液中的CUS NP进行阳离子交换反应。所得Cu2 +可以使用Poly T DNA作为模板形成荧光Cu NPS。 Cu NP的荧光强度可用于确定靶DNA的浓度。为了进一步提高检测灵敏度，使用两种类型的DNA装饰磁珠。在EXO III消化后，两个循环过程，更多的CUS NP进入上清液。因此，在阳离子交换反应和荧光Cu nps的形成后发现了更强的荧光强度。开发方法方便，低成本，灵敏度良好和选择性。

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  • 来源
    《RSC Advances》 |2018年第27期|共5页
  • 作者

    Song Weiling; Zhang Nan; Luan Zhenzhu; Zhang Xiaoru; He Peng;

  • 作者单位

    Qingdao Univ Sci &

    Technol Key Lab Sensor Anal Tumor Marker Minist Educ Coll Chem &

    Mol Engn Qingdao 266042 Peoples R China;

    Qingdao Univ Sci &

    Technol Key Lab Sensor Anal Tumor Marker Minist Educ Coll Chem &

    Mol Engn Qingdao 266042 Peoples R China;

    Qingdao Univ Sci &

    Technol Key Lab Sensor Anal Tumor Marker Minist Educ Coll Chem &

    Mol Engn Qingdao 266042 Peoples R China;

    Qingdao Univ Sci &

    Technol Key Lab Sensor Anal Tumor Marker Minist Educ Coll Chem &

    Mol Engn Qingdao 266042 Peoples R China;

    Qingdao Univ Sci &

    Technol Key Lab Sensor Anal Tumor Marker Minist Educ Coll Chem &

    Mol Engn Qingdao 266042 Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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