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首页> 外文期刊>RSC Advances >The polysaccharide of Atractylodes macrocephala koidz (PAMK) alleviates cyclophosphamide-mediated immunosuppression in geese, possibly through novel_mir2 targeting of CTLA4 to upregulate the TCR-NFAT pathway
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The polysaccharide of Atractylodes macrocephala koidz (PAMK) alleviates cyclophosphamide-mediated immunosuppression in geese, possibly through novel_mir2 targeting of CTLA4 to upregulate the TCR-NFAT pathway

机译：Atractylodes macrocephala Koidz（PAMK）的多糖可缓解鹅中的环磷酰胺介导的免疫抑制，可能通过CTLA4的Novel _mir2靶向上调TCR-NFAT途径

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摘要

The polysaccharide of Atractylodes macrocephala koidz (PAMK) has been proved to have antioxidant, antiinflammatory, antiviral, and immunity promoting effects. MicroRNAs (miRNAs) have also been shown to participate in the regulation of immune function by negatively regulating the expression of target genes. However, little is known about how PAMK alleviates the immunosuppression via the miRNA pathway in geese. The aim of this study is to evaluate the influence of PAMK on immunosuppression. Magang geese (1 day old, n = 200) were randomly divided into groups, namely, the control group (normal feeding), PAMK (fed 400 mg kg(-1) PAMK), CTX (injected 40 mg kg(-1) BW cyclophosphamide), and CTX + PAMK (40 mg kg(-1) BW cyclophosphamide + 400 mg kg(-1) PAMK) groups. Thymus development was examined by the thymus index, transmission electron microscopy and scanning electron microscopy. The T cell proliferation rate was stimulated by phytoagglutinin (PHA), and T cell activation related genes (CD28, CD96, MHC-II), and IL-2 levels in serum were detected. Differentially expressed miRNAs of geese to regulate T cell activation were found by miRNA sequencing technologies. The results showed that PAMK could alleviate thymus damage and the decrease in the T lymphocyte proliferation rate, T cell activation, and IL-2 levels that were induced by CTX. MiRNA sequencing found that the combination of PAMK and CTX significantly promoted T cell activation via upregulation of novel_mir2 (P 0.05), which inhibited cytotoxic T lymphocyte antigen 4 (CTLA4) expressions, thereby promoting the TCR-NFAT signaling pathway. It can be concluded that PAMK, through novel_mir2 targeting of CTLA4 to upregulate TCR pathway, finally alleviated immunosuppression induced by CTX in geese.
机译：已证明Atractylode Macrocephala Koidz（PAMK）的多糖具有抗氧化剂，抗炎，抗病毒和免疫促进效果。还证明了MicroRNAs（miRNA）通过对靶基因的表达产生负面调节靶基因的表达来参与免疫功能的调节。然而，关于PAMK如何通过鹅中的miRNA途径减轻免疫抑制的知识众所周知。本研究的目的是评估PAMK对免疫抑制的影响。 Magang Geese（1天大，N = 200）被随机分为组，即对照组（正常喂养），PAMK（喂食400mg kg（-1）pamk），CTX（注射40 mg kg（-1） BW环磷酰胺）和CTX + PAMK（40mg kg（-1）bw环磷酰胺+ 400mg kg（-1）pamk）组。通过胸腺指数，透射电子显微镜和扫描电子显微镜检查胸腺发育。通过植物血糖素（PHA）刺激T细胞增殖率，检测T细胞活化相关基因（CD28，CD96，MHC-II）和血清中的IL-2水平。 MiRNA测序技术发现肠鹅的MiRNA来调节T细胞活化。结果表明，CTX诱导的T淋巴细胞增殖率，T细胞活化和IL-2水平可缓解胸腺损伤及降低。 MiRNA测序发现，PAMK和CTX的组合通过新出的新_mir2（p <0.05）的上调显着促进了T细胞活化，其抑制细胞毒性T淋巴细胞抗原4（CTLA4）表达，从而促进TCR-NFAT信号通路。可以得出结论，通过NefoL_mir2靶向CTLA4来上调TCR途径，最终缓解了CTX在鹅中的免疫抑制。

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  • 来源
    《RSC Advances》 |2018年第47期|共12页
  • 作者

    Li Wanyan; Xu Danning; Li Bingxin; Cao Nan; Guo Sixuan; Jiang Qingyan; Tian Yunbo;

  • 作者单位

    Guangdong Prov Key Lab Waterfowl Hlth Breeding Guangzhou 510225 Guangdong Peoples R China;

    Guangdong Prov Key Lab Waterfowl Hlth Breeding Guangzhou 510225 Guangdong Peoples R China;

    Guangdong Prov Key Lab Waterfowl Hlth Breeding Guangzhou 510225 Guangdong Peoples R China;

    Guangdong Prov Key Lab Waterfowl Hlth Breeding Guangzhou 510225 Guangdong Peoples R China;

    Guangdong Prov Key Lab Waterfowl Hlth Breeding Guangzhou 510225 Guangdong Peoples R China;

    South China Agr Univ Coll Anim Sci Guangdong Prov Key Lab Anim Nutr Control Guangzhou 510642 Guangdong Peoples R China;

    Guangdong Prov Key Lab Waterfowl Hlth Breeding Guangzhou 510225 Guangdong Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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