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首页> 外文期刊>RSC Advances >Grafted neural stem cells show lesion-specific migration in radiation-injured rat brains
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Grafted neural stem cells show lesion-specific migration in radiation-injured rat brains

机译：嫁接神经干细胞显示出辐射损伤大鼠大脑的病变特异性迁移

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摘要

Neural stem cells (NSCs) exhibit preferential homing toward some types of brain lesion, but their migratory property during radiation brain injury (RBI) remains unexplored. Here, we use the superparamagnetic iron oxide (SPIO)-labeled magnetic resonance imaging (MRI) technology to determine the migration of transplanted NSCs in two partial RBI models in real time, created by administering 30-55 Gy of radiation to the right or posterior half of the adult rat brain. SPIO-labeled NSCs were stereotactically grafted into the uninjured side one week after RBI. The migration of SPIO-labeled NSCs in live radiation-injured brains was traced by MRI for up to 28 days after engraftment and quantified for their moving distances and speeds. A high labeling efficiency (>90%) was achieved by incubating NSCs with 100 mu g ml(-1) of SPIO for 12-24 hours. Upon stereotactic transplantation into the healthy side of the brain, SPIO-labeled NSCs were distinctively detected as hypointense signals on T2-weighted images (T2WI), showed sustained survival for up to 4 weeks, and exhibited directional migration to the radiation-injured side of the brain with a speed of 86-127 mm per day. The moving kinetics of grafted NSCs displayed no difference in brains receiving a high (55 Gy) vs. moderate (45 Gy) dose of radiation, but was slower in the right RBI model than in the posterior RBI model. This study shows that NSCs can be effectively labeled by SPIO and traced in vivo by MRI, and that grafted NSCs exhibit directional migration toward RBI sites in a route-dependent but radiation dose-independent manner.
机译：神经干细胞（NSCs）表现出对某些类型的脑病变的优先归巢，但在辐射脑损伤（RBI）期间的迁移性仍未探索。在这里，我们使用超顺磁性氧化铁（SPIO） - 标记的磁共振成像（MRI）技术实时地确定在两个部分RBI模型中移植的NSCs迁移，通过向右或后部施用30-55GY辐射来创造成年大鼠的一半。在RBI之后一周，Spio标记的NSCs立刻嫁接到未加注的一侧。在植入后的MRI追踪活辐射损伤的大脑中的Spio标记的NSCs的迁移，可在植入后28天追踪，并为其移动距离和速度量化。通过将NSCs与100μgml（-1）的Spio孵育12-24小时来实现高标记效率（> 90％）。在立体定向移植到大脑的健康方面，将SPIO-标记的NSCs独特地检测为T2加权图像（T2WI）上的低音信号，显示出长达4周的持续存活，并向辐射损伤的侧向迁移大脑每天速度为86-127毫米。接枝NSC的移动动力学显示出接受高（55Gy）与中等（45μg）剂量的辐射的大脑中没有差异，但在右侧RBI模型中较慢。该研究表明，NSCs可以通过SPIO有效地标记并通过MRI追踪体内，并且接枝的NSCs在依赖于途径的辐射剂量的辐射剂量的辐射辐射辐射的辐射剂量的情况下表现出向RBI位点的定向迁移。

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  • 来源
    《RSC Advances》 |2018年第11期|共9页
  • 作者

    Bai Shou-Min; Wang Qiong; Yu Xiao-Li; Chen Ting; Yang Jin; Shi Jun-Tian; Tsai Robert Y. L.; Huang Hai;

  • 作者单位

    Sun Yat Sen Univ Sun Yat Sen Mem Hosp Dept Radiat Oncol Guangzhou 510120 Guangdong Peoples R China;

    Sun Yat Sen Univ Sun Yat Sen Mem Hosp Dept Urol Guangzhou 510120 Guangdong Peoples R China;

    Sun Yat Sen Univ Sun Yat Sen Mem Hosp Dept Radiat Oncol Guangzhou 510120 Guangdong Peoples R China;

    Sun Yat Sen Univ Sun Yat Sen Mem Hosp Dept Radiat Oncol Guangzhou 510120 Guangdong Peoples R China;

    Sun Yat Sen Univ Sun Yat Sen Mem Hosp Dept Radiat Oncol Guangzhou 510120 Guangdong Peoples R China;

    Sun Yat Sen Univ Sun Yat Sen Mem Hosp Dept Radiat Oncol Guangzhou 510120 Guangdong Peoples R China;

    Texas A&

    M Univ Hlth Sci Ctr Inst Biosci &

    Technol Houston TX 77030 USA;

    Sun Yat Sen Univ Sun Yat Sen Mem Hosp Dept Urol Guangzhou 510120 Guangdong Peoples R China;

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  • 中图分类 化学;
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