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首页> 外文期刊>RSC Advances >Transcriptome analysis reveals GA induced apoptosis in HCT116 human colon cancer cells through calcium and p53 signal pathways
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Transcriptome analysis reveals GA induced apoptosis in HCT116 human colon cancer cells through calcium and p53 signal pathways

机译：转录组分析揭示了通过钙和P53信号途径在HCT116人结肠癌细胞中诱导凋亡

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摘要

Gallic acid (GA) is a polyphenol widely found in numerous fruits and vegetables that has been reported to exert anticancer effects, including apoptosis, against cancer cell lines. However, little is known about the induction of apoptotic effects and the underlying mechanism. We used RNA-seq to examine differentially expressed genes in human colon cancer HCT116 cells after 12 h and 24 h exposure to GA. A total of 792 and 911 genes with known functions showed significantly different expression levels in 12 h and 24 h GA-treated HCT116 cells, respectively. KEGG enrichment analysis showed that the identified genes were involved in pathways such as cholinergic synapse, circadian entrainment, calcium signal processing and transport, arachidonic acid metabolism and the p53 signal pathway. Real-time quantitative PCR was used to validate the reliability of the results obtained by RNA-seq. The results of this study indicate that GA triggers apoptosis in HCT116 cells through obstructing the growth of cells in the early phase treatment by down-regulation of calcium channels and then up-regulation of the intrinsic p53 signal pathway through activation of apoptosis caspases, finally leading to the mitochondrial apoptosis pathway.
机译：Gallic acid（Ga）是一种广泛发现的多酚，据报道，据报道，患有对癌细胞系的抗癌作用，包括细胞凋亡。然而，关于凋亡效应和潜在机制的诱导很少。我们使用RNA-SEQ在12小时和24小时暴露于GA后检查人结肠癌HCT116细胞中的差异表达基因。共有792和911个具有已知功能的基因分别在12小时和24小时治疗的HCT116细胞中显示出显着不同的表达水平。 Kegg浓缩分析表明，鉴定的基因涉及胆碱能突触，昼夜夹带，钙信号处理和运输，花生酸代谢和P53信号途径等途径。实时定量PCR用于验证RNA-SEQ获得的结果的可靠性。该研究的结果表明，通过阻碍通过钙通道的下调，通过激活细胞凋亡的Caspases来阻碍早期相位处理中细胞的生长，通过激活细胞凋亡酶，通过激活细胞凋亡酶的抑制细胞的生长，表明GA触发HCT116细胞中的细胞凋亡。对线粒体细胞凋亡途径。

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  • 来源
    《RSC Advances》 |2018年第22期|共10页
  • 作者

    Yang Chen; Xie Xiaoyu; Tang Hu; Dong Xuyan; Zhang Xiaodong; Huang Fenghong;

  • 作者单位

    Chinese Acad Agr Sci Oil Crops Res Inst Wuhan 430062 Hubei Peoples R China;

    Wuhan Univ Coll Life Sci Dept Cell Biol Wuhan 430072 Hubei Peoples R China;

    Chinese Acad Agr Sci Oil Crops Res Inst Wuhan 430062 Hubei Peoples R China;

    Chinese Acad Agr Sci Oil Crops Res Inst Wuhan 430062 Hubei Peoples R China;

    Wuhan Univ Coll Life Sci Dept Cell Biol Wuhan 430072 Hubei Peoples R China;

    Chinese Acad Agr Sci Oil Crops Res Inst Wuhan 430062 Hubei Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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