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Rapid naked-eye detection of Gram-positive bacteria by vancomycin-based nano-aggregation

机译：基于Vancomycin的纳米聚集的革兰氏阳性细菌的快速肉眼检测

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摘要

Development of a rapid, point-of-care assay for diagnosing bacterial infections is crucial for subsequent treatment of the patient and preventing the overuse of antibiotics. Herein, we describe a rapid, one-step colorimetric assay based on the formation of nano-aggregates using nanobeads targeting Gram-positive bacteria. Vancomycin was immobilized onto blue-colored polymeric nanobeads to induce specific and multivalent binding with the Gram-positive bacterial cell wall and subsequent agglomeration. Without any pre-processing steps, the addition of various types of Gram-positive pathogens to the nanobeads resulted in the formation of blue precipitates, which could be observed with the naked eye in approximate to 30 min. We also utilized a porous filter system for the assay, which allowed discrimination of Gram-positive targets with higher selectivity, and demonstrated feasibility as a simple diagnostic assay with minimal technical components. We anticipate that the nanobead aggregation assay can be potentially applied as a rapid and simple sensing platform, which can be easily miniaturized and enable point-of-care diagnosis of Gram-positive infections.
机译：用于诊断细菌感染的快速，护理测定的开发对于随后治疗患者并防止过度使用抗生素是至关重要的。在此，我们基于使用靶向革兰氏阳性细菌的纳米形状的纳米聚集体的形成快速的一步比色测定。使万古霉素固定在蓝色聚合物纳米珠上，以诱导与革兰氏阳性细菌细胞壁和随后的附聚结合的特异性和多价结合。在没有任何预处理步骤的情况下，将各种类型的克阳性病原体添加到纳米罩中导致蓝色沉淀物的形成，其可以用肉眼观察到近30分钟。我们还利用了一种用于测定的多孔过滤系统，其允许鉴别具有更高的选择性的革兰氏阳性靶标，并表现为具有最小技术部件的简单诊断测定的可行性。我们预期纳米型聚集测定可以作为快速和简单的传感平台潜在应用，这可以很容易地小型化并能够实现革兰阳性感染的护理点诊断。

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  • 来源
    《RSC Advances》 |2018年第44期|共10页
  • 作者

    Shin Cheong; Lee Ha Neul; Ryu Jea Sung; Chung Hyun Jung;

  • 作者单位

    Korea Adv Inst Sci &

    Technol Grad Sch Nanosci &

    Technol 291 Univ Rd Daejeon 34141 South Korea;

    Korea Adv Inst Sci &

    Technol Grad Sch Nanosci &

    Technol 291 Univ Rd Daejeon 34141 South Korea;

    Korea Adv Inst Sci &

    Technol Grad Sch Nanosci &

    Technol 291 Univ Rd Daejeon 34141 South Korea;

    Korea Adv Inst Sci &

    Technol Grad Sch Nanosci &

    Technol 291 Univ Rd Daejeon 34141 South Korea;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
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