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Design of a gold nanoprobe for the detection of Pseudomonas aeruginosa elastase gene (lasB)

机译：用于检测假单胞菌铜绿假单胞菌酶基因（LASB）的金纳骨孔的设计

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摘要

Nosocomial infections are one of the major health problems that increase mortality. Pseudomonas aeruginosa is one of the important causes of nosocomial infections. This bacterium has a gene called the lasB gene, the product of which is a zinc-dependent metalloprotease. This gene plays a central role in the pathogenesis of Pseudomonas spp. This pathogen is highly toxic and destroys tissues and also has a moderating effect on the immune system; on the other hand, it initiates the intracellular pathway of biofilm growth. Although there are methods such as molecular methods for identifying the lasB gene, due to the high cost and the need for specialized personnel, it is necessary to replace them with an appropriate method. In this study, a gold nanoparticle-based DNA diagnostic sensor sensitive to the aggregation states of gold nanoparticles was used to identify amplified and non-amplified lasB genes. The results of the experiment were evaluated both visually and spectrally. The minimum detection value of this method was 10 ng of the amplified lasB gene and 50 ng of the non-amplified lasB gene. This method is very fast, simple, easy and low cost.
机译：医院感染是增加死亡率的主要健康问题之一。假单胞菌铜绿假单胞菌是医院感染的重要原因之一。该细菌具有称为LASB基因的基因，其产品是锌依赖性金属蛋白酶。该基因在伪霉素SPP的发病机制中起着重要作用。该病原体具有高毒性和破坏组织，并且还对免疫系统具有调节效果;另一方面，它引发了生物膜生长的细胞内途径。尽管存在诸如用于鉴定LASB基因的分子方法等方法，但由于高成本和对专业人员的需求，因此必须以适当的方法更换它们。在该研究中，使用与金纳米粒子的聚集状态敏感的基于金纳米粒子的DNA诊断传感器鉴定扩增和未扩增的LASB基因。实验结果在视觉和光谱上进行评估。该方法的最小检测值为10ng扩增的LASB基因和50ng的未扩增的LASb基因。这种方法非常快，简单，简单，成本低。

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  • 来源
    《RSC Advances》 |2020年第20期|共8页
  • 作者

    Farhangi Alireza; Peymani Amir; Ahmadpour-Yazdi Hossien;

  • 作者单位

    Qazvin Univ Med Sci Student Res Comm Qazvin Iran;

    Qazvin Univ Med Sci Med Microbiol Res Ctr Qazvin Iran;

    Qazvin Univ Med Sci Fac Paramed Sci Med Biotechnol Dept Qazvin Iran;

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  • 正文语种 eng
  • 中图分类 化学;
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