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High-throughput in-focus differential interference contrast imaging of three-dimensional orientations of single gold nanorods coated with a mesoporous silica shell

机译:涂有介孔二氧化硅壳的单金纳墩三维取向的高通量聚焦差分干扰成像

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摘要

Plasmonic gold nanorods (AuNRs) have been widely applied as optical orientation probes in many biophysical studies. However, characterizing the various three-dimensional (3D) orientations of AuNRs in the same focal plane of the objective lens is a challenging task. To overcome this challenge, we fabricated single AuNRs (10 nm x 30 nm) coated with either an elliptical or spherical mesoporous silica shell (AuNRs@mSiO(2)). Unlike bare AuNRs and elliptical AuNRs@mSiO(2), spherical AuNRs@mSiO(2)contained randomly oriented AuNR cores in 3D space, which could be observed on the same focal plane within a single frame by differential interference contrast (DIC) microscopy. The spherical AuNRs@mSiO(2)thus achieved high-throughput detection. The proposed approach can overcome the limitations of the current gel-matrix method, which requires vertical scanning of the embedded AuNRs to capture different focal planes.
机译:在许多生物物理学研究中,等离子体金纳米棒(AUNRS)被广泛应用于光学取向探针。 然而,表征在物镜的相同焦平面中的AUNR的各种三维(3D)取向是一个具有挑战性的任务。 为了克服这一挑战,我们制造了涂有椭圆形或球形介孔二氧化硅壳(AUNRS @ MSIO(2))的单个AUNR(10nm x 30 nm)。 与裸AUNR和椭圆形AUNRS不同于MSIO(2),球面AUNRS @ MSIO(2)包含在3D空间中的随机定向的AUNR核心,可以通过差分干扰对比度(DIC)显微镜在单个帧内的同一个焦平面上观察到。 由此实现了球形AUNRS @ MSIO(2)实现了高通量检测。 所提出的方法可以克服当前凝胶矩阵方法的局限性,这需要嵌入式AUNR的垂直扫描以捕获不同的焦平面。

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  • 来源
    《RSC Advances》 |2020年第50期|共5页
  • 作者单位

    Univ Ulsan Dept Chem 93 Daehak Ro Ulsan 44610 South Korea;

    Sungkyunkwan Univ SKKU Res Ctr Adv Mat Technol SKKU Adv Inst Nanotechnol SAINT Suwon 16419 South Korea;

    Sungkyunkwan Univ SKKU Res Ctr Adv Mat Technol SKKU Adv Inst Nanotechnol SAINT Suwon 16419 South Korea;

    Univ Ulsan Dept Chem 93 Daehak Ro Ulsan 44610 South Korea;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
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