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Selection and application of aptamers with high-affinity and high-specificity against dinophysistoxin-1

机译：具有高亲和力和高特异性的适体选择和应用针对灌注药毒素-1

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摘要

Diarrhetic shellfish toxins (DSTs) are marine toxins distributed widely in the world, which pose a major threat to the health of mankind. Dinophysistoxin-1 (DTX-1) has the most potent toxicity in DSTs. However, the current detection methods have ethical problems and technical defects. Further research is needed, to develop a more suitable alternative to the supervision system. In this work, we successfully obtained an aptamer with high affinity and specificity bound to DTX-1 for the first time. After optimization, a core sequence of the aptamer with a higher K-D of 64 nM was obtained, while the binding mode of the core sequence and DTX-1 was explored. Based on this aptamer, we developed a biolayer interferometry (BLI) biosensor platform for DTX-1 detection. The aptasensor exhibited a broad detection range from 40 to 600 nM DTX-1 (linear range from 80 to 200 nM), and the low detection limit was 614 pM. Morever, the aptasensor showed good reproducibility and stability, which indicated that this novel aptasensor had broad development prospects for the sensitive and rapid detection of DTX-1.
机译：腹泻贝类毒素（DSTS）是海洋毒素在世界范围内广泛分布，对人类健康构成了重大威胁。 Dinophysistoxin-1（DTX-1）在DST中具有最有效的毒性。然而，目前的检测方法具有道德问题和技术缺陷。需要进一步研究，为监督系统制定更合适的替代品。在这项工作中，我们首次成功获得了具有高亲和力和特异性的适体和特异性。优化后，获得具有64nm的k-d较高的k-d的适体的核心序列，而探讨了核序列和DTX-1的结合模式。基于该适体，我们开发了一种用于DTX-1检测的Biolayer干涉测量（BLI）生物传感器平台。 Aptasensor的宽度检测范围为40至600nm DTX-1（线性范围为80至200nm），低检测限为614ppm。 Morever，Aptasensor显示出良好的再现性和稳定性，这表明这种新的Aptasensor具有广泛的开发前景，用于DTX-1的敏感和快速检测。

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  • 来源
    《RSC Advances》 |2020年第14期|共9页
  • 作者

    Li Zhen; Hu Bo; Zhou Rong; Zhang Xiaojuan; Wang Ruizhe; Gao Yun; Sun Mingjuan; Jiao Binghua; Wang Lianghua;

  • 作者单位

    Navy Med Univ Coll Basic Med Sci Dept Biochem &

    Mol Biol Shanghai 200433 Peoples R China;

    Navy Med Univ Coll Basic Med Sci Dept Biochem &

    Mol Biol Shanghai 200433 Peoples R China;

    Navy Med Univ Coll Basic Med Sci Dept Biochem &

    Mol Biol Shanghai 200433 Peoples R China;

    Navy Med Univ Coll Basic Med Sci Dept Biochem &

    Mol Biol Shanghai 200433 Peoples R China;

    Second Mil Med Univ Changzheng Hosp Dept Orthoped Spine Ctr 415th Feng Yang Rd Shanghai 200003 Peoples R China;

    Navy Med Univ Coll Basic Med Sci Dept Biochem &

    Mol Biol Shanghai 200433 Peoples R China;

    Navy Med Univ Coll Basic Med Sci Dept Biochem &

    Mol Biol Shanghai 200433 Peoples R China;

    Navy Med Univ Coll Basic Med Sci Dept Biochem &

    Mol Biol Shanghai 200433 Peoples R China;

    Navy Med Univ Coll Basic Med Sci Dept Biochem &

    Mol Biol Shanghai 200433 Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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