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首页> 外文期刊>RSC Advances >Metabolic characterisation ofMagnetospirillum gryphiswaldenseMSR-1 using LC-MS-based metabolite profiling
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Metabolic characterisation ofMagnetospirillum gryphiswaldenseMSR-1 using LC-MS-based metabolite profiling

机译：使用基于LC-MS的代谢物分析，Magnetospirillum gryphiswaldensemsr-1代谢表征

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摘要

Magnetosomes are nano-sized magnetic nanoparticles with exquisite properties that can be used in a wide range of healthcare and biotechnological applications. They are biosynthesised by magnetotactic bacteria (MTB), such asMagnetospirillum gryphiswaldenseMSR-1 (Mgryph). However, magnetosome bioprocessing yields low quantities compared to chemical synthesis of magnetic nanoparticles. Therefore, an understanding of the intracellular metabolites and metabolic networks related toMgryphgrowth and magnetosome formation are vital to unlock the potential of this organism to develop improved bioprocesses. In this work, we investigated the metabolism ofMgryphusing untargeted metabolomics. Liquid chromatography-mass spectrometry (LC-MS) was performed to profile spent medium samples ofMgryphcells grown under O-2-limited (n= 6) and O-2-rich conditions (n= 6) corresponding to magnetosome- and non-magnetosome producing cells, respectively. Multivariate, univariate and pathway enrichment analyses were conducted to identify significantly altered metabolites and pathways. Rigorous metabolite identification was carried out using authentic standards, theMgryph-specific metabolite database and MS/MS mzCloud database. PCA and OPLS-DA showed clear separation and clustering of sample groups with cross-validation values of (RX)-X-2 = 0.76, (RY)-Y-2 = 0.99 and Q(2)= 0.98 in OPLS-DA. As a result, 50 metabolites linked to 45 metabolic pathways were found to be significantly altered in the tested conditions, including: glycine, serine and threonine; butanoate; alanine, aspartate and glutamate metabolism; aminoacyl-tRNA biosynthesis and; pyruvate and citric acid cycle (TCA) metabolisms. Our findings demonstrate the potential of LC-MS to characterise key metabolites inMgryphand will contribute to further understanding the metabolic mechanisms that affectMgryphgrowth and magnetosome formation.
机译：磁性体是纳米大小的磁性纳米粒子，具有精致的性能，可用于各种医疗保健和生物技术应用。它们是通过磁直流细菌（MTB）的生物合成，例如Magnetospirillum GryphiswaldensemsR-1（Murgorph）。然而，与磁性纳米粒子的化学合成相比，磁体生物处理产生低量。因此，理解细胞内代谢物和代谢网络相关的Tomgryphrowth和磁体形成对于解锁这种有机体的潜力来开发改善的生物处理。在这项工作中，我们调查了对未确定的代谢组学的Mgretphusing的新陈代谢。进行液相色谱 - 质谱（LC-MS）对对应于磁体和非磁体的O-2限制（n = 6）和O-2富含条件（n = 6）的型血液中的介质样品进行分析介质样品。分别产生细胞。进行多元，单变量和途径富集分析以确定显着改变的代谢物和途径。使用真实标准，特定于HOTEMGROM代谢物数据库和MS / MS MZCLOUD数据库进行严格的代谢物识别。 PCA和OPLS-DA显示OPLS-DA中的交叉验证值（RX）-X-2 = 0.76，（RY）-Y-2 = 0.99和Q（2）= 0.98的交叉验证值的清晰分离和聚类。结果，发现50种与45个代谢途径相关的代谢物在测试的条件下显着改变，包括：甘氨酸，丝氨酸和苏氨酸;丁酸盐;丙氨酸，天冬氨酸和谷氨酸代谢;氨基酰基 - TRNA生物合成和;丙酮酸和柠檬酸循环（TCA）代谢。我们的研究结果证明了LC-MS的潜力，以表征关键代谢物InmgryPhand将有助于进一步了解影响影响的代谢机制和磁体形成。

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  • 来源
    《RSC Advances》 |2020年第54期|共13页
  • 作者

    Abdelrazig Salah; Safo Laudina; Rance Graham A.; Fay Michael W.; Theodosiou Eirini; Topham Paul D.; Kim Dong-Hyun; Fernandez-Castane Alfred;

  • 作者单位

    Univ Nottingham Sch Pharm Ctr Analyt Biosci Adv Mat &

    Healthcare Technol Div Nottingham NG7 2RD England;

    Univ Nottingham Sch Pharm Ctr Analyt Biosci Adv Mat &

    Healthcare Technol Div Nottingham NG7 2RD England;

    Univ Nottingham Nanoscale &

    Microscale Res Ctr Nottingham NG7 2RD England;

    Univ Nottingham Nanoscale &

    Microscale Res Ctr Nottingham NG7 2RD England;

    Aston Univ Aston Inst Mat Res Birmingham B4 7ET W Midlands England;

    Aston Univ Aston Inst Mat Res Birmingham B4 7ET W Midlands England;

    Univ Nottingham Sch Pharm Ctr Analyt Biosci Adv Mat &

    Healthcare Technol Div Nottingham NG7 2RD England;

    Aston Univ Aston Inst Mat Res Birmingham B4 7ET W Midlands England;

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  • 正文语种 eng
  • 中图分类 化学;
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