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Fabrication of low-fouling, high-loading polymeric surfaces through pH-controlled RAFT

机译：通过pH控制筏制造低污垢，高负载聚合物表面

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摘要

Low-fouling and high-loading surfaces are increasingly important for biosensing and blood purification technologies. Selective and efficient target binding from complex media can be achieved with poly(carboxybetaine) (pCB) surfaces that consist of a dense brush layer to resist non-specific protein adsorption and a sparse "mushroom" upper layer for high-density capture agent immobilization (i.e. high-loading). We developed pH-controlled surface-reversible addition-fragmentation chain-transfer (S-RAFT) polymerization to simplify fabrication of multi-modal, low-fouling and high-loading pCB surfaces without the need for quenching or re-initiation steps, toxic transition metals or light irradiation. Multi-modal polymer layers were produced through partial polymer termination by temporarily raising the pH to aminolyse a fraction of dormant chain transfer agents (CTAs); remaining polymer chains with intact CTAs continued uninterrupted extension to create the "mushroom" upper layer. The multi-modal pCB surfaces were low-fouling towards proteins (<6.7 ng cm(-2)), and macrophages. Compared to mono-modal brush surfaces, multi-modal pCB surfaces were high-loading with 5-fold greater capture agent immobilization (e.g. antibody) and 4-fold greater target binding (e.g. biotin-fluorescein).
机译：低污垢和高负载表面对于生物腐蚀和血液净化技术越来越重要。来自复合介质的选择性和有效的靶结合可以通过由致密刷层组成的聚（羧基脲）（PCB）表面来实现，以抵抗非特异性蛋白质吸附和稀疏的“蘑菇”上层，用于高密度捕获剂固定（即高负荷）。我们开发了pH控制的表面可逆添加 - 碎片链转移（S-RAFT）聚合，以简化多模态，低污垢和高负荷PCB表面的制造，而无需淬火或重新启动步骤，有毒转变金属或光照射。通过临时提高pH以静脉分离的休眠链转移剂（CTA）通过部分聚合物终止产生多模态聚合物层;剩余的聚合物链具有完整的CTA，继续不间断地延伸以产生“蘑菇”上层。多模态PCB表面朝向蛋白质（<6.7ng cm（-2））和巨噬细胞是低污染的。与单模型刷表面相比，多模态PCB表面具有5倍捕获剂固定（例如抗体）和4倍的靶结合（例如Biotin-Fluorescin）的高负载。

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  • 来源
    《RSC Advances》 |2020年第34期|共11页
  • 作者

    Jesmer Alexander H.; Huynh Vincent; Wylie Ryan G.;

  • 作者单位

    McMaster Univ Dept Chem &

    Chem Biol Hamilton ON L8S 4M1 Canada;

    McMaster Univ Dept Chem &

    Chem Biol Hamilton ON L8S 4M1 Canada;

    McMaster Univ Dept Chem &

    Chem Biol Hamilton ON L8S 4M1 Canada;

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  • 正文语种 eng
  • 中图分类 化学;
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