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Properties of differentiated SH-SY5Y grown on carbon-based materials

机译：碳基材料上种植的差异化SH-SY5Y的性质

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摘要

Neural cell differentiation has been extensively studied in two-dimensional (2D) cell culture plates. However, the cellular microenvironment and extracellular matrix (ECM) are much more complex and flat 2D surfaces are hard to mimic in ECM. Carbon nanotubes (CNTs) and graphenes are multidimensional carbon-based nanomaterials and may be able to provide extra dimensions on cell growth and differentiation. To determine the effect of CNTs and graphene surfaces on the growth, gene expression, differentiation and functionality of neuroblastoma to a neural cell, SH-SY5Y cells were grown on a 2D (control) surface, a CNT network and a graphene film. The data suggest that SH-SY5Y cells grown on CNT surfaces show an average 20.2% increase in cell viability; 5.7% decrease in the ratio of cells undergoing apoptosis; 78.3, 43.4 and 38.1% increases in SOX2, GFAP and NeuN expression, respectively; and a 29.7% increase in mean firing rate on a multi-electrode array. SH-SY5Y cells grown on graphene film show little or no changes in cell properties compared to cells grown in 2D. The data indicate that the three-dimensional (3D) surface of CNTs provides a favorable environment for SH-SY5Y cells to proliferate and differentiate to neurons.
机译：在二维（2D）细胞培养板中已经广泛研究了神经细胞分化。然而，细胞微环境和细胞外基质（ECM）更复杂，并且在ECM中难以模拟平坦的2D表面。碳纳米管（CNT）和石墨烯是多维碳基纳米材料，并且可以在细胞生长和分化中提供额外的尺寸。为了确定CNT和石墨烯表面对神经母细胞瘤的生长，基因表达，分化和功能的影响，在2D（对照）表面，CNT网络和石墨烯膜上生长SH-SY5Y细胞。数据表明，在CNT表面上生长的SH-SY5Y细胞显示平均细胞活力增加20.2％;在进行细胞凋亡的细胞比例下降5.7％; 78.3,43.4和38.1％分别增加了SOX2，GFAP和Neun表达;在多电极阵列上的平均射击率增加29.7％。与2D中生长的细胞相比，在石墨烯薄膜上生长的SH-SY5Y细胞显示很少或没有细胞性质的变化。数据表明CNT的三维（3D）表面为SH-SY5Y细胞提供了有利的环境，以增殖和分化为神经元。

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  • 来源
    《RSC Advances》 |2020年第33期|共8页
  • 作者

    Yoon Sae-Bom; Lee Geonhee; Park Sung Bum; Cho Heeyeong; Lee Jeong-O; Koh Byumseok;

  • 作者单位

    Korea Res Inst Chem Technol Biotechnol &

    Therapeut Div 141 Gajeong Ro Daejeon 34114 South Korea;

    Korea Res Inst Chem Technol Adv Mat Div 141 Gajeong Ro Daejeon 34114 South Korea;

    Korea Res Inst Chem Technol Biotechnol &

    Therapeut Div 141 Gajeong Ro Daejeon 34114 South Korea;

    Korea Res Inst Chem Technol Biotechnol &

    Therapeut Div 141 Gajeong Ro Daejeon 34114 South Korea;

    Korea Res Inst Chem Technol Adv Mat Div 141 Gajeong Ro Daejeon 34114 South Korea;

    Korea Res Inst Chem Technol Biotechnol &

    Therapeut Div 141 Gajeong Ro Daejeon 34114 South Korea;

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  • 正文语种 eng
  • 中图分类 化学;
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